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FOXP2 (NM_148899) Human Over-expression Lysate

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SKU
LC407756
Lyophilized FOXP2 HEK293T cell transient overexpression lysate (as WB positive control)
Size:
20 ug 100 ug
Formulation:
Lyophilized Liquid
  $206.00
2 Weeks*
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC215759
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_148899, NP_683697
Synonyms CAGH44; SPCH1; TNRC10
Predicted MW 70.1 kDa
Components 1 vial of 20 ug lyophilized total protein derived from gene specific transient over-expression cell lysate
Storage The lysate can be shipped at ambient temperature, however upon receipt of the sample store at -20°C. Lysate samples are stable for 12 months from the date of receipt when stored at -20°C in their original lyophilized form.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C. Lysate samples are stable for 12 months from date of receipt when stored at -20°C.
Shipping Blue Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution Method The 20 µg lyophilized sample can be reconstituted with deionized water, or directly into 1x SDS-PAGE running buffer (with reducing agent) if the sample is being prepared directly to be run on a gel.  The lyophilized 20 ug product is derived from a liquid solution at 1 mg/ml of total lysate protein, thus reconstituting back to 1 mg/ml using 20 µl is recommended.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C.
Reference Data
RefSeq NP_683697
Locus ID 93986
Cytogenetics 7q31.1
Protein Families WB
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Other "FOXP2" proteins (6)
SKU Description Size Price
PH315021 FOXP2 MS Standard C13 and N15-labeled recombinant protein (NP_055306) 10 ug
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LC415248 Lyophilized FOXP2 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
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LY407756 Transient overexpression lysate of forkhead box P2 (FOXP2), transcript variant 3 (as WB positive control) 100 ug
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LY415248 Transient overexpression lysate of forkhead box P2 (FOXP2), transcript variant 1 (as WB positive control) 100 ug
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TP315021 Recombinant protein of human forkhead box P2 (FOXP2), transcript variant 1, 20 µg 20 ug
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TP761959 Purified recombinant protein of Human forkhead box P2 (FOXP2), transcript variant 3,full length, with N-terminal GST and C-terminal His tag, expressed in E. coli, 50ug 50 ug
$270.00

file-alt Citations

Parallel immunizations of rabbits using the same antigen yield antibodies with similar, but not identical, epitopes
Hjelm, Forsström, Löfblom et al
PLoS ONE (2012) 7 (12), e45817 DOI: 10.1371/journal.pone.0045817
Cited by 19
AI Summary: In this study, OriGene’s FOXP2 over-expressing HEK293T cell lysate (product LY407756) was used as a source of antigen to independently validate the specificity of the generated polyclonal antibodies.
Snippet: of the antibodies against six of the targets with an independent assay (Table 1). Therefore, 15 amino acid long synthetic peptides ... Uppsala, Sweden)), liver tissue and one cell lysate of HEK293T cells over-expressing the target protein FOXP2 (LY407756, Origene Technologies).
FigureFigureFigure
Showing 1-1 of 1 papers.
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