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CLEC14A (NM_175060) Human Over-expression Lysate

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SKU
LC406300
Lyophilized CLEC14A HEK293T cell transient overexpression lysate (as WB positive control)
Size:
20 ug 100 ug
Formulation:
Lyophilized Liquid
  $206.00
2 Weeks*
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC206655
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_175060, NP_778230
Synonyms C14orf27; CEG1; EGFR-5
Predicted MW 51.6 kDa
Components 1 vial of 20 ug lyophilized total protein derived from gene specific transient over-expression cell lysate
Storage The lysate can be shipped at ambient temperature, however upon receipt of the sample store at -20°C. Lysate samples are stable for 12 months from the date of receipt when stored at -20°C in their original lyophilized form.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C. Lysate samples are stable for 12 months from date of receipt when stored at -20°C.
Shipping Blue Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution Method The 20 µg lyophilized sample can be reconstituted with deionized water, or directly into 1x SDS-PAGE running buffer (with reducing agent) if the sample is being prepared directly to be run on a gel.  The lyophilized 20 ug product is derived from a liquid solution at 1 mg/ml of total lysate protein, thus reconstituting back to 1 mg/ml using 20 µl is recommended.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C.
Reference Data
RefSeq NP_778230
Locus ID 161198
Cytogenetics 14q21.1
Protein Families WB
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Other "CLEC14A" proteins (1)
SKU Description Size Price
LY406300 Transient overexpression lysate of C-type lectin domain family 14, member A (CLEC14A) (as WB positive control) 100 ug
$436.00

file-alt Citations

Methylation of CLEC14A is associated with its expression and lung adenocarcinoma progression
Su, Shi, Cheng et al
J. Cell. Physiol. (2018)
AI Summary: OriGene’s PWPXL‐CLEC14A plasmid was used to overexpress CLEC14A in lung adenocarcinoma cell lines, enabling the authors to study the gene’s role in cell proliferation, apoptosis, migration, invasion, and tumorigenicity.
Snippet: 2.8 Transfection. PWPXL‐CLEC14A (Origene, China) was used for transfection experiments. Cells were transfected by use of Lipofectamine LTX reagent (Invitrogen) as recommended by the manufacturer. G418 treatment (Invitrogen) selected stably transfected cells
Sprouting angiogenesis is regulated by shedding of the C-type lectin family 14, member A (CLEC14A) ectodomain, catalyzed by rhomboid-like 2 protein (RHBDL2)
Noy, Swain, Khan et al
FASEB J. (2016)
AI Summary: OriGene's hCLEC14A IMAGE clone was used as the PCR template to generate constructs for studying the role of CLEC14A in angiogenesis in this paper.
Snippet: For pEGFPn1 HA-hCLEC14A, first pEGFPn1 hCLEC14A was generated by PCR from a hCLEC14A IMAGE clone (Origene, Rockville, MD, USA), EcoR1 sites were in- troduced at the 59 and 39 ends of the fragment and cloned into this site in the pEGFP-n1 plasmid ( ... Cite SaveSaving
Blocking CLEC14A-MMRN2 binding inhibits sprouting angiogenesis and tumour growth
Noy, Lodhia, Khan et al
Oncogene (2015)
Cited by 61
AI Summary: OriGene’s clec14a IMAGE clone was used as the source for generating CLEC14A-Fc fusion constructs, enabling the study of CLEC14A’s role and its interaction with MMRN2 in angiogenesis and tumor growth.
Snippet: IRES-EGFP;Addgene) were used. pWPI hCLEC14A-Fc and mCLEC14A-Fc was generated by initial PCR subcloning from clec14a IMAGE clone (Origene, Rockville, MD, USA) into pcDNA3-Fc plasmid. The primers used were ... Cite SaveSaving
Showing 1-3 of 3 papers.
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