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Dicer (DICER1) (NM_177438) Human Over-expression Lysate

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SKU
LC406165
Lyophilized DICER1 HEK293T cell transient overexpression lysate (as WB positive control)
Size:
20 ug 100 ug
Formulation:
Lyophilized Liquid
  $206.00
2 Weeks*
Bulk/Customize

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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC219214
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_177438, NP_803187
Synonyms DCR1; Dicer; Dicer1e; GLOW; HERNA; K12H4.8-LIKE; MNG1; RMSE2
Predicted MW 218.5 kDa
Components 1 vial of 20 ug lyophilized total protein derived from gene specific transient over-expression cell lysate
Storage The lysate can be shipped at ambient temperature, however upon receipt of the sample store at -20°C. Lysate samples are stable for 12 months from the date of receipt when stored at -20°C in their original lyophilized form.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C. Lysate samples are stable for 12 months from date of receipt when stored at -20°C.
Shipping Blue Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution Method The 20 µg lyophilized sample can be reconstituted with deionized water, or directly into 1x SDS-PAGE running buffer (with reducing agent) if the sample is being prepared directly to be run on a gel.  The lyophilized 20 ug product is derived from a liquid solution at 1 mg/ml of total lysate protein, thus reconstituting back to 1 mg/ml using 20 µl is recommended.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C.
Reference Data
RefSeq NP_803187
Locus ID 23405
Cytogenetics 14q32.13
Protein Families WB
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Other "Dicer" proteins (7)
SKU Description Size Price
PH311804 DICER1 MS Standard C13 and N15-labeled recombinant protein (NP_085124) 10 ug
$3,360.00
PH319214 DICER1 MS Standard C13 and N15-labeled recombinant protein (NP_803187) 10 ug
$3,360.00
LC410795 Lyophilized DICER1 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LY406165 Transient overexpression lysate of dicer 1, ribonuclease type III (DICER1), transcript variant 1 (as WB positive control) 100 ug
$665.00
LY410795 Transient overexpression lysate of dicer 1, ribonuclease type III (DICER1), transcript variant 2 (as WB positive control) 100 ug
$665.00
TP311804 Purified recombinant protein of Homo sapiens dicer 1, ribonuclease type III (DICER1), transcript variant 2, 20 µg 20 ug
$867.00
TP319214 Recombinant protein of human dicer 1, ribonuclease type III (DICER1), transcript variant 1, 20 µg 20 ug
$867.00

file-alt Citations

Aging-induced dysregulation of dicer1-dependent microRNA expression impairs angiogenic capacity of rat cerebromicrovascular endothelial cells
Ungvari, Tucsek, Sosnowska et al
J. Gerontol. A Biol. Sci. Med. Sci. (2013) 68 (8), 877-91 DOI: 10.1093/gerona/gls242
Cited by 139
AI Summary: OriGene’s proprietary Dicer1 shRNA sequences were used to downregulate Dicer1 expression in cerebromicrovascular endothelial cells, enabling the study of how reduced Dicer1 impairs angiogenic capacity and miRNA regulation in aging.
Snippet: MicroRNAs (miRNAs) are short, endogenous, noncoding transcripts that negatively regulate the expression of specific mRNA targets ... CMVECs, Dicer1 was downregulated (by ~50%) by RNA interference using four proprietary shRNA sequences (OriGene Technologies, Rockville
Showing 1-1 of 1 papers.
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