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FAM83A (NM_207006) Human Over-expression Lysate

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SKU
LC404130
Lyophilized FAM83A HEK293T cell transient overexpression lysate (as WB positive control)
Size:
20 ug 100 ug
Formulation:
Lyophilized Liquid
  $206.00
2 Weeks*
Bulk/Customize

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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC219879
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_207006, NP_996889
Synonyms BJ-TSA-9
Predicted MW 40.4 kDa
Components 1 vial of 20 ug lyophilized total protein derived from gene specific transient over-expression cell lysate
Storage The lysate can be shipped at ambient temperature, however upon receipt of the sample store at -20°C. Lysate samples are stable for 12 months from the date of receipt when stored at -20°C in their original lyophilized form.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C. Lysate samples are stable for 12 months from date of receipt when stored at -20°C.
Shipping Blue Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution Method The 20 µg lyophilized sample can be reconstituted with deionized water, or directly into 1x SDS-PAGE running buffer (with reducing agent) if the sample is being prepared directly to be run on a gel.  The lyophilized 20 ug product is derived from a liquid solution at 1 mg/ml of total lysate protein, thus reconstituting back to 1 mg/ml using 20 µl is recommended.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C.
Reference Data
RefSeq NP_996889
Locus ID 84985
Cytogenetics 8q24.13
Protein Families WB
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Other "FAM83A" proteins (5)
SKU Description Size Price
PH308565 FAM83A MS Standard C13 and N15-labeled recombinant protein (NP_116288) 10 ug
$3,360.00
LC409861 Lyophilized FAM83A HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LY404130 Transient overexpression lysate of family with sequence similarity 83, member A (FAM83A), transcript variant 2 (as WB positive control) 100 ug
$436.00
LY409861 Transient overexpression lysate of family with sequence similarity 83, member A (FAM83A), transcript variant 1 (as WB positive control) 100 ug
$436.00
TP308565 Recombinant protein of human family with sequence similarity 83, member A (FAM83A), transcript variant 1, 20 µg 20 ug
$867.00

file-alt Citations

HER2-positive breast cancer cells expressing elevated FAM83A are sensitive to FAM83A loss
Bartel, Jackson
PLoS ONE (2017) 12 (5), e0176778 DOI: 10.1371/journal.pone.0176778
AI Summary: OriGene’s TissueScan Breast Cancer Panels 1-4 were used to examine FAM83A expression levels in HER2-positive breast cancer samples, contributing to the identification of elevated FAM83A in a subset of tumors.
Snippet: trastuzumab. Materials and Methods. FAM83A expression in HER2+ breast cancer. FAM83A expression was examined in HER2+ breast cancer using TCGA data TissueScan Breast Cancer Panels 1-4 (Origene Technologies, Inc). ... All 6 versions Cite SaveSaving
FigureFigureFigure
Showing 1-1 of 1 papers.
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