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LRG1 (NM_052972) Human Over-expression Lysate

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SKU
LC403277
Lyophilized LRG1 HEK293T cell transient overexpression lysate (as WB positive control)
Size:
20 ug 100 ug
Formulation:
Lyophilized Liquid
  $206.00
2 Weeks*
Bulk/Customize

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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC206780
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_052972, NP_443204
Synonyms HMFT1766; LRG
Predicted MW 38.2 kDa
Components 1 vial of 20 ug lyophilized total protein derived from gene specific transient over-expression cell lysate
Storage The lysate can be shipped at ambient temperature, however upon receipt of the sample store at -20°C. Lysate samples are stable for 12 months from the date of receipt when stored at -20°C in their original lyophilized form.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C. Lysate samples are stable for 12 months from date of receipt when stored at -20°C.
Shipping Blue Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution Method The 20 µg lyophilized sample can be reconstituted with deionized water, or directly into 1x SDS-PAGE running buffer (with reducing agent) if the sample is being prepared directly to be run on a gel.  The lyophilized 20 ug product is derived from a liquid solution at 1 mg/ml of total lysate protein, thus reconstituting back to 1 mg/ml using 20 µl is recommended.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C.
Reference Data
RefSeq NP_443204
Locus ID 116844
Cytogenetics 19p13.3
Protein Families WB
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Other "LRG1" proteins (4)
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file-alt Citations

Discovery of lung cancer biomarkers by profiling the plasma proteome with monoclonal antibody libraries
Guergova-Kuras, Kurucz, Hempel et al
Mol. Cell Proteomics (2011) 10 (12), M111.010298 DOI: 10.1074/mcp.M111.010298
Cited by 8
AI Summary: OriGene’s True-Clone plasmid ORIG-SC321789 was used to transfect HEK293T cells, enabling the expression of target proteins necessary for validating lung cancer biomarkers identified in the study.
Snippet: 3) and showed improved survival (4), because of the relatively low specificity of CT imaging, there is an imminent need for better ... HEK293T cells at ∼60% confluency were transfected with the plasmid, ORIG-SC321789, from OriGene (Rockville, MD) containing the True-Clone
FigureFigureFigure
Showing 1-1 of 1 papers.
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