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FOXP1 (NM_032682) Human Over-expression Lysate

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SKU
LC403191
Lyophilized FOXP1 HEK293T cell transient overexpression lysate (as WB positive control)
Size:
20 ug 100 ug
Formulation:
Lyophilized Liquid
  $206.00
In Stock*
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC213862
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_032682, NP_116071
Synonyms 12CC4; hFKH1B; HSPC215; MFH; QRF1
Predicted MW 75.1 kDa
Components 1 vial of 20 ug lyophilized total protein derived from gene specific transient over-expression cell lysate
Storage The lysate can be shipped at ambient temperature, however upon receipt of the sample store at -20°C. Lysate samples are stable for 12 months from the date of receipt when stored at -20°C in their original lyophilized form.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C. Lysate samples are stable for 12 months from date of receipt when stored at -20°C.
Shipping Blue Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution Method The 20 µg lyophilized sample can be reconstituted with deionized water, or directly into 1x SDS-PAGE running buffer (with reducing agent) if the sample is being prepared directly to be run on a gel.  The lyophilized 20 ug product is derived from a liquid solution at 1 mg/ml of total lysate protein, thus reconstituting back to 1 mg/ml using 20 µl is recommended.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C.
Reference Data
RefSeq NP_116071
Locus ID 27086
Cytogenetics 3p13
Protein Families WB
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Other "FOXP1" proteins (4)
SKU Description Size Price
PH313862 FOXP1 MS Standard C13 and N15-labeled recombinant protein (NP_116071) 10 ug
$3,360.00
LY403191 Transient overexpression lysate of forkhead box P1 (FOXP1), transcript variant 1 (as WB positive control) 100 ug
$665.00
TP313862 Recombinant protein of human forkhead box P1 (FOXP1), transcript variant 1, 20 µg 20 ug
$867.00
TP710030 Recombinant protein of human forkhead box P1 (FOXP1), transcript variant 1,full length, with C-terminal DDK tag, expressed in sf9 cells 20 ug
$540.00

file-alt Citations

Identification of a de novo FOXP1 mutation and incidental discovery of inherited genetic variants contributing to a case of autism spectrum disorder and epilepsy
Jay, Mitra, Harding et al
Mol Genet Genomic Med (2019) 7 (7), e00751 DOI: 10.1002/mgg3.751
AI Summary: In this study, the FOXP1 overexpression lysate (LC40319, OriGene) was used as a positive control in Western blot experiments to validate FOXP1 protein detection.
Snippet: Western blots were completed using antibodies for FOXP1 (D35D10, Cell Signaling Technology) and PTEN (D4.3, CST), with β‐actin (8H10D10, CST) used as a loading control, and a FOXP1 overexpression lysate (LC40319, OriGene) used as a posi- tive control
FoxP1 Stimulates Angiogenesis by Repressing the Inhibitory Guidance Protein Semaphorin 5B in Endothelial Cells
Grundmann, Lindmayer, Hans et al
PLoS ONE (2013) 8 (9), e70873 DOI: 10.1371/journal.pone.0070873
Cited by 37
AI Summary: OriGene products contributed to this paper by providing the human semaphorin 5B ORF clone (NM_001031702.2) and the control construct pCMV6-XL5, which were used to investigate the regulatory relationship between FoxP1 and semaphorin 5B in endothelial cells.
Snippet: ORF clone of human semaphorin 5B (NM_001031702.2) and the appropriate control constructs (pCMV6-XL5) were obtained from Origene Technologies (Rockville, MD). ... and FoxP1b) that exist in the zebrafish and resulted in either the generation of a mis-spliced band (MO1) or
FigureFigureFigure
Showing 1-2 of 2 papers.
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