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DKK3 (NM_015881) Human Over-expression Lysate

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SKU
LC402468
Lyophilized DKK3 HEK293T cell transient overexpression lysate (as WB positive control)
Size:
20 ug 100 ug
Formulation:
Lyophilized Liquid
  $206.00
2 Weeks*
Bulk/Customize

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Other products for "DKK3 or NM_015881"

Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC221022
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_015881, NP_056965
Synonyms REIC; RIG
Predicted MW 38.3 kDa
Components 1 vial of 20 ug lyophilized total protein derived from gene specific transient over-expression cell lysate
Storage The lysate can be shipped at ambient temperature, however upon receipt of the sample store at -20°C. Lysate samples are stable for 12 months from the date of receipt when stored at -20°C in their original lyophilized form.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C. Lysate samples are stable for 12 months from date of receipt when stored at -20°C.
Shipping Blue Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution Method The 20 µg lyophilized sample can be reconstituted with deionized water, or directly into 1x SDS-PAGE running buffer (with reducing agent) if the sample is being prepared directly to be run on a gel.  The lyophilized 20 ug product is derived from a liquid solution at 1 mg/ml of total lysate protein, thus reconstituting back to 1 mg/ml using 20 µl is recommended.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C.
Reference Data
RefSeq NP_056965
Locus ID 27122
Cytogenetics 11p15.3
Protein Families WB
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Other "DKK3" proteins (8)
SKU Description Size Price
PH321022 DKK3 MS Standard C13 and N15-labeled recombinant protein (NP_056965) 10 ug
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LC415712 Lyophilized DKK3 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC422711 Lyophilized DKK3 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
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LY402468 Transient overexpression lysate of dickkopf homolog 3 (Xenopus laevis) (DKK3), transcript variant 1 (as WB positive control) 100 ug
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LY415712 Transient overexpression lysate of dickkopf homolog 3 (Xenopus laevis) (DKK3), transcript variant 2 (as WB positive control) 100 ug
$436.00
LY422711 Transient overexpression lysate of dickkopf homolog 3 (Xenopus laevis) (DKK3), transcript variant 3 (as WB positive control) 100 ug
$436.00
TP321022 Recombinant protein of human dickkopf homolog 3 (Xenopus laevis) (DKK3), transcript variant 1, 20 µg 20 ug
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TP720348 Recombinant protein of human dickkopf homolog 3 (Xenopus laevis) (DKK3), transcript variant 3 10 ug
$235.00

file-alt Citations

microRNA-183 is an oncogene targeting Dkk-3 and SMAD4 in prostate cancer
Ueno, Hirata, Shahryari et al
Br. J. Cancer (2013) 108 (8), 1659-67 DOI: 10.1038/bjc.2013.125
Cited by 120
AI Summary: OriGene products, specifically the PrecisionShuttle pCMV6-Entry Vector and the Myc-DDK-tagged ORF clone of human Dkk-3 (NM_015881.5), were used as transfection-ready DNA constructs to study the functional role and regulation of Dkk-3 by miR-183 in prostate cancer cells.
Snippet: Plasmid. PrecisionShuttle pCMV6-Entry Vector and Myc-DDK-tagged ORF clone of homo sapiens dickkopf homolog-3 (Xenopus laevis, Dkk-3), transcript variant 1 as transfection-ready DNA NM_015881.5 were purchased from ORIGENE (Rockville, MD, USA).
Wnt antagonist DICKKOPF-3 (Dkk-3) induces apoptosis in human renal cell carcinoma
Ueno, Hirata, Majid et al
Mol. Carcinog. (2011) 50 (6), 449-57 DOI: 10.1002/mc.20729
Cited by 61
AI Summary: OriGene’s transfection-ready DNA for the human jun oncogene (JUN, NM_002228.3) was used in this study to investigate the molecular mechanisms by which Dkk-3 induces apoptosis in renal cell carcinoma cells.
Snippet: well tissue culture plate and transfected with 250 ng of pCMV6-Entry Vector or Homo sapiens jun oncogene (JUN) as transfection-ready DNA NM_002228.3 (ORIGENE), 237.5 ng ... 13 Tsuji T, Miyazaki M, Sakaguchi M, Inoue Y, Namba M. A REIC gene shows down-regulation in
Showing 1-2 of 2 papers.
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