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SLC14A1 (NM_015865) Human Over-expression Lysate

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SKU
LC402465
Lyophilized SLC14A1 HEK293T cell transient overexpression lysate (as WB positive control)
Size:
20 ug 100 ug
Formulation:
Lyophilized Liquid
  $206.00
2 Weeks*
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC208509
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_015865, NP_056949
Synonyms HsT1341; HUT11; JK; Jk(b); RACH1; RACH2; UT-B1; UT1; UTE
Predicted MW 42.5 kDa
Components 1 vial of 20 ug lyophilized total protein derived from gene specific transient over-expression cell lysate
Storage The lysate can be shipped at ambient temperature, however upon receipt of the sample store at -20°C. Lysate samples are stable for 12 months from the date of receipt when stored at -20°C in their original lyophilized form.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C. Lysate samples are stable for 12 months from date of receipt when stored at -20°C.
Shipping Blue Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution Method The 20 µg lyophilized sample can be reconstituted with deionized water, or directly into 1x SDS-PAGE running buffer (with reducing agent) if the sample is being prepared directly to be run on a gel.  The lyophilized 20 ug product is derived from a liquid solution at 1 mg/ml of total lysate protein, thus reconstituting back to 1 mg/ml using 20 µl is recommended.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C.
Reference Data
RefSeq NP_056949
Locus ID 6563
Cytogenetics 18q12.3
Protein Families WB
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Other "SLC14A1" proteins (9)
SKU Description Size Price
PH308509 SLC14A1 MS Standard C13 and N15-labeled recombinant protein (NP_056949) 10 ug
$3,360.00
LC426961 Lyophilized SLC14A1 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC431384 Lyophilized SLC14A1 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
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LC431897 Lyophilized SLC14A1 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LY402465 Transient overexpression lysate of solute carrier family 14 (urea transporter), member 1 (Kidd blood group) (SLC14A1), transcript variant 2 (as WB positive control) 100 ug
$436.00
LY426961 Transient overexpression lysate of solute carrier family 14 (urea transporter), member 1 (Kidd blood group) (SLC14A1), transcript variant 1 (as WB positive control) 100 ug
$436.00
LY431384 Transient overexpression lysate of solute carrier family 14 (urea transporter), member 1 (Kidd blood group) (SLC14A1), transcript variant 4 (as WB positive control) 100 ug
$436.00
LY431897 Transient overexpression lysate of solute carrier family 14 (urea transporter), member 1 (Kidd blood group) (SLC14A1), transcript variant 3 (as WB positive control) 100 ug
$436.00
TP308509 Recombinant protein of human solute carrier family 14 (urea transporter), member 1 (Kidd blood group) (SLC14A1), transcript variant 2, 20 µg 20 ug
$737.00

file-alt Citations

Expression and localization of a UT-B urea transporter in the human bladder
Walpole, Farrell, McGrane et al
Am. J. Physiol. Renal Physiol. (2014)
Cited by 26
AI Summary: OriGene products—including purified human UT-B1 protein (TP308509), UT-B1 whole cell lysate (LY402465), and UT-B2 whole cell lysate (LY426961)—were used as controls and references in immunoblotting experiments to confirm the presence and characterize the size of UT-B proteins in human bladder tissue.
Snippet: Page 6. Immunoblotting 104 Purified human UT-B1 protein (TP308509, Origene Technologies Inc., USA), UT- 105 B1 whole cell lysate (LY402465, Origene Technologies Inc., USA) and UT-B2 whole cell 106 lysate (LY426961, Origene Technologies Inc., USA) were ... Cite SaveSaving
Showing 1-1 of 1 papers.
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