SLC27A6 (NM_014031) Human Over-expression Lysate
SKU
LC402279
Lyophilized SLC27A6 HEK293T cell transient overexpression lysate (as WB positive control)
$206.00
2 Weeks*
Specifications
| Product Data | |
| Species | Human |
|---|---|
| Expression Host | HEK293T |
| Expression cDNA Clone or AA Sequence | TrueORF Clone RC207410 |
| Tag | C-Myc/DDK |
| Detection Antibodies | Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100) |
| ACCN | NM_014031, NP_054750 |
| Synonyms | ACSVL2; FACVL2; FATP6; VLCS-H1 |
| Predicted MW | 70.1 kDa |
| Components | 1 vial of 20 ug lyophilized total protein derived from gene specific transient over-expression cell lysate |
| Storage | The lysate can be shipped at ambient temperature, however upon receipt of the sample store at -20°C. Lysate samples are stable for 12 months from the date of receipt when stored at -20°C in their original lyophilized form. After reconstitution, avoid repeat freeze thaw cycles and store at -80°C. Lysate samples are stable for 12 months from date of receipt when stored at -20°C. |
| Shipping | Blue Ice |
| Preparation | HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins. |
| Reconstitution Method | The 20 µg lyophilized sample can be reconstituted with deionized water, or directly into 1x SDS-PAGE running buffer (with reducing agent) if the sample is being prepared directly to be run on a gel. The lyophilized 20 ug product is derived from a liquid solution at 1 mg/ml of total lysate protein, thus reconstituting back to 1 mg/ml using 20 µl is recommended. After reconstitution, avoid repeat freeze thaw cycles and store at -80°C. |
| Reference Data | |
| RefSeq | NP_054750 |
| Locus ID | 28965 |
| Cytogenetics | 5q23.3 |
| Protein Families | WB |
| Protein Pathways | PPAR signaling pathway |
Reviews
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Resources
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Other "SLC27A6" proteins (3)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| LC422766 | Lyophilized SLC27A6 HEK293T cell transient overexpression lysate (as WB positive control) | 20 ug |
$206.00
|
|
| LY402279 | Transient overexpression lysate of solute carrier family 27 (fatty acid transporter), member 6 (SLC27A6), transcript variant 1 (as WB positive control) | 100 ug |
$436.00
|
|
| LY422766 | Transient overexpression lysate of solute carrier family 27 (fatty acid transporter), member 6 (SLC27A6), transcript variant 2 (as WB positive control) | 100 ug |
$665.00
|
|
Citations
Downregulation of SLC27A6 by DNA Hypermethylation Promotes Proliferation but Suppresses Metastasis of Nasopharyngeal Carcinoma Through Modulating Lipid Metabolism
Zhong, Yang, Li et al
Front Oncol (2021) 11, 780410 DOI: 10.3389/fonc.2021.780410
Zhong, Yang, Li et al
Front Oncol (2021) 11, 780410 DOI: 10.3389/fonc.2021.780410
Cited by 28
AI Summary: In this study, OriGene products including the SLC27A6 expression plasmid (RC211307) and SLC27A6-specific qPCR primers (HQP022612) were used to manipulate and measure SLC27A6 expression in nasopharyngeal carcinoma cells, enabling the authors to elucidate the gene's role in tumor cell proliferation, metastasis, and lipid metabolism.
Snippet: …Solute carrier family 27 member 6 (SLC27A6) mediates the cellular uptake of long-chain fatty acid (LCFA), a necessary lipid component. However, the functions of SLC27A6 in NPC remain unknown. Here, we found a significant reduction of SLC27A6 mRNA in NPC tissues compared with normal nasopharyngeal epithelia (NNE). The promoter methylation ratio of SLC27A6 was greater in NPC than in non-cancerous tissues. The demethylation reagent 5-aza-2'-deoxycytidine (5-aza-dC) remarkably restored the mRNA expression of SLC27A6, suggesting that this gene was downregulated in NPC owing to DNA promoter hypermethylation. Furthermore, SLC27A6 overexpression level in NPC cell lines led to significant suppression of cell proliferation, clonogenicity in vitro, and tumorigenesis in vivo. Higher SLC27A6 expression, on the other hand, promoted NPC cell migration and invasion. In particular, re-expression of SLC27A6 faciliated epithelial-mesenchymal transition (EMT) signals in xenograft tumors. Furthermore, we observed that SLC27A6 enhanced the intracellular amount of triglyceride (TG) and total cholesterol (T-CHO) in NPC cells, contributing to lipid biosynthesis and increasing metastatic potential. Notably, the mRNA level of SLC27A6 was positively correlated with cancer stem cell (CSC) markers, CD24 and CD44. In summary, DNA promoter hypermethylation downregulated the expression of SLC27A6. Furthermore, re-expression of SLC27A6 inhibited the growth capacity of NPC cells but strengthened the CSC markers. Our findings revealed the dual role of SLC27A6 in NPC and shed novel light on the link between lipid metabolism and CSC maintenance.Copyright 2022 Zhong, Yang, Li, Liang, Huang, Zheng, Wang, Xiao, Mo, Zhang, Zhou, Huang and Zhao
Showing 1-1 of 1 papers.
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