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GPR4 (NM_005282) Human Over-expression Lysate

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SKU
LC401625
Lyophilized GPR4 HEK293T cell transient overexpression lysate (as WB positive control)
Size:
20 ug 100 ug
Formulation:
Lyophilized Liquid
  $206.00
2 Weeks*
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC209686
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_005282, NP_005273
Synonyms GPR6C.l
Predicted MW 40.9 kDa
Components 1 vial of 20 ug lyophilized total protein derived from gene specific transient over-expression cell lysate
Storage The lysate can be shipped at ambient temperature, however upon receipt of the sample store at -20°C. Lysate samples are stable for 12 months from the date of receipt when stored at -20°C in their original lyophilized form.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C. Lysate samples are stable for 12 months from date of receipt when stored at -20°C.
Shipping Blue Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution Method The 20 µg lyophilized sample can be reconstituted with deionized water, or directly into 1x SDS-PAGE running buffer (with reducing agent) if the sample is being prepared directly to be run on a gel.  The lyophilized 20 ug product is derived from a liquid solution at 1 mg/ml of total lysate protein, thus reconstituting back to 1 mg/ml using 20 µl is recommended.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C.
Reference Data
RefSeq NP_005273
Locus ID 2828
Cytogenetics 19q13.32
Protein Families WB
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Documents
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Other "GPR4" proteins (1)
SKU Description Size Price
LY401625 Transient overexpression lysate of G protein-coupled receptor 4 (GPR4) (as WB positive control) 100 ug
$436.00

file-alt Citations

Short-chain fatty acids and acidic pH up-regulate UT-B, GPR41, and GPR4 in rumen epithelial cells of goats
Lu, Gui, Yao et al
Am. J. Physiol. Regul. Integr. Comp. Physiol. (2014)
Cited by 56
AI Summary: OriGene’s HEK293T cell lysate, known to overexpress GPR4, was used as a positive control to validate GPR4 protein detection in the study.
Snippet: lysate of human HEK293T cell line (OriGene, MD, USA) was used, since HEK293T cells are known 214 to overexpress the GPR4 protein. ... Page 12. human endothelial cells (45) and similar to the band of GPR4 predicted by the manufactory (OriGene, 218 MD, USA). ... Cite SaveSaving
Showing 1-1 of 1 papers.
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