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MAGE 1 (MAGEA1) (NM_004988) Human Over-expression Lysate

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SKU
LC401552
Lyophilized MAGEA1 HEK293T cell transient overexpression lysate (as WB positive control)
Size:
20 ug 100 ug
Formulation:
Lyophilized Liquid
  $206.00
2 Weeks*
Bulk/Customize

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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC202134
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_004988, NP_004979
Synonyms CT1.1; MAGE1
Predicted MW 34.3 kDa
Components 1 vial of 20 ug lyophilized total protein derived from gene specific transient over-expression cell lysate
Storage The lysate can be shipped at ambient temperature, however upon receipt of the sample store at -20°C. Lysate samples are stable for 12 months from the date of receipt when stored at -20°C in their original lyophilized form.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C. Lysate samples are stable for 12 months from date of receipt when stored at -20°C.
Shipping Blue Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution Method The 20 µg lyophilized sample can be reconstituted with deionized water, or directly into 1x SDS-PAGE running buffer (with reducing agent) if the sample is being prepared directly to be run on a gel.  The lyophilized 20 ug product is derived from a liquid solution at 1 mg/ml of total lysate protein, thus reconstituting back to 1 mg/ml using 20 µl is recommended.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C.
Reference Data
RefSeq NP_004979
Locus ID 4100
Cytogenetics Xq28
Protein Families WB
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Other "MAGE 1" proteins (3)
SKU Description Size Price
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LY401552 Transient overexpression lysate of melanoma antigen family A, 1 (directs expression of antigen MZ2-E) (MAGEA1) (as WB positive control) 100 ug
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file-alt Citations

Post-transcriptional regulation of cancer/testis antigen MAGEC2 expression by TRIM28 in tumor cells
Song, Guo, Zheng et al
BMC Cancer (2018) 18 (1), 971 DOI: 10.1186/s12885-018-4844-1
AI Summary: OriGene’s anti-MAGEA3/6 antibody (TA800918) was used in this study to detect MAGEA3/6 protein levels by Western blotting, contributing to the analysis of MAGEC2 regulation in tumor cells.
Snippet: Antibodies used in this study were as follows: anti-GAPDH (AP0066, Bioworld, USA), anti-β-actin (AP0060, Bioworld), anti-TRIM28 (#4124, CST, USA;MB0014, Bioworld), anti-MAGEA1 (BS1217, Bioworld), anti-MAGEA3/6 (TA800918, Origene, USA), anti-MAGEA10 Cached
FigureFigureFigure
WB IHC IP
Showing 1-1 of 1 papers.
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