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SLUG (SNAI2) (NM_003068) Human Over-expression Lysate

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SKU
LC401070
Lyophilized SNAI2 HEK293T cell transient overexpression lysate (as WB positive control)
Size:
20 ug 100 ug
Formulation:
Lyophilized Liquid
  $206.00
2 Weeks*
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC202365
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_003068, NP_003059
Synonyms SLUG; SLUGH; SLUGH1; SNAIL2; WS2D
Predicted MW 29.8 kDa
Components 1 vial of 20 ug lyophilized total protein derived from gene specific transient over-expression cell lysate
Storage The lysate can be shipped at ambient temperature, however upon receipt of the sample store at -20°C. Lysate samples are stable for 12 months from the date of receipt when stored at -20°C in their original lyophilized form.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C. Lysate samples are stable for 12 months from date of receipt when stored at -20°C.
Shipping Blue Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution Method The 20 µg lyophilized sample can be reconstituted with deionized water, or directly into 1x SDS-PAGE running buffer (with reducing agent) if the sample is being prepared directly to be run on a gel.  The lyophilized 20 ug product is derived from a liquid solution at 1 mg/ml of total lysate protein, thus reconstituting back to 1 mg/ml using 20 µl is recommended.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C.
Reference Data
RefSeq NP_003059
Locus ID 6591
Cytogenetics 8q11.21
Protein Families WB
Protein Pathways Adherens junction
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Other "SLUG" proteins (2)
SKU Description Size Price
LY401070 Transient overexpression lysate of snail homolog 2 (Drosophila) (SNAI2) (as WB positive control) 100 ug
$436.00
TP760008 Recombinant protein of human snail homolog 2 (Drosophila) (SNAI2), full length, with N-terminal HIS tag, expressed in E.Coli, 50ug 50 ug
$270.00

file-alt Citations

Snail coordinately regulates downstream pathways to control multiple aspects of Mammalian neural precursor development
Zander, Burns, Yang et al
J. Neurosci. (2014) 34 (15), 5164-75 DOI: 10.1523/JNEUROSCI.0370-14.2014
AI Summary: OriGene products contributed murine Cdc25b and Snail2 expression constructs, which were used to study the roles of these genes in regulating survival, proliferation, and differentiation of mammalian neural precursor cells.
Snippet: The p53 shRNA was kindly provided by Dr. Reuven Agami's laboratory (Netherlands Cancer Institute) (Brummelkamp et al., 2002). Murine Cdc25b and Snail2 expression constructs were purchased from Origene. Cortical precursor cell cultures. ... Cite SaveSaving
Showing 1-1 of 1 papers.
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