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Oct4 (POU5F1) (NM_002701) Human Over-expression Lysate

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SKU
LC400950
Lyophilized POU5F1 HEK293T cell transient overexpression lysate (as WB positive control)
Size:
20 ug 100 ug
Formulation:
Lyophilized Liquid
  $206.00
2 Weeks*
Bulk/Customize

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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC211998
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_002701, NP_002692
Synonyms Oct-3; Oct-4; OCT3; OCT4; OTF-3; OTF3; OTF4
Predicted MW 38.4 kDa
Components 1 vial of 20 ug lyophilized total protein derived from gene specific transient over-expression cell lysate
Storage The lysate can be shipped at ambient temperature, however upon receipt of the sample store at -20°C. Lysate samples are stable for 12 months from the date of receipt when stored at -20°C in their original lyophilized form.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C. Lysate samples are stable for 12 months from date of receipt when stored at -20°C.
Shipping Blue Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution Method The 20 µg lyophilized sample can be reconstituted with deionized water, or directly into 1x SDS-PAGE running buffer (with reducing agent) if the sample is being prepared directly to be run on a gel.  The lyophilized 20 ug product is derived from a liquid solution at 1 mg/ml of total lysate protein, thus reconstituting back to 1 mg/ml using 20 µl is recommended.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C.
Reference Data
RefSeq NP_002692
Locus ID 5460
Cytogenetics 6p21.33
Protein Families WB
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Other "Oct4" proteins (6)
SKU Description Size Price
PH311998 POU5F1 MS Standard C13 and N15-labeled recombinant protein (NP_002692) 10 ug
$3,360.00
LC404373 Lyophilized POU5F1 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LY400950 Transient overexpression lysate of POU class 5 homeobox 1 (POU5F1), transcript variant 1 (as WB positive control) 100 ug
$436.00
LY404373 Transient overexpression lysate of POU class 5 homeobox 1 (POU5F1), transcript variant 2 (as WB positive control) 100 ug
$436.00
TP311998 Recombinant protein of human POU class 5 homeobox 1 (POU5F1), transcript variant 1, 20 µg 20 ug
$867.00
TP760938 Purified recombinant protein of Human POU class 5 homeobox 1 (POU5F1/OCT4), transcript variant 2, full length, with N-terminal HIS tag, expressed in E. coli, 50ug 50 ug
$270.00

file-alt Citations

FOLFOX Therapy Induces Feedback Upregulation of CD44v6 through YB-1 to Maintain Stemness in Colon Initiating Cells
Ghatak, Hascall, Markwald et al
Int J Mol Sci (2021) 22 (2) DOI: 10.3390/ijms22020753
Cited by 27
AI Summary: In this study, OriGene products, specifically the CD44v6 (Cat# SC317380) and YB-1 (Cat# SR303548) CRISPR/Cas9 knockout plasmids, were used to delete CD44v6 and YB-1 genes, enabling the authors to demonstrate their roles in maintaining stemness and drug resistance in colon cancer initiating cells.
Snippet: [36], and aldehyde dehydrogenase (ALDH1) [37] describe CIC characteristics. However, the cell-surface markers that recognize CICs and have a functional role in the antiapoptotic signaling to drive tumorigenesis have remained poorly defined. Our working hypothesis for this study is that CICs are involved in the development of drug-resistance, treatment failure, and tumor relapse in cancer [38,39]
FigureFigureFigure
A systematic approach to quantitative Western blot analysis
Pillai-Kastoori, Schutz-Geschwender, Harford
Anal Biochem (2020) 593, 113608 DOI: 10.1016/j.ab.2020.113608
Cited by 426
AI Summary: OriGene products, specifically the β-Actin (TA-09) and GAPDH (TA-08) antibodies, were used in this study as loading controls to validate and ensure the accuracy of quantitative Western blot analysis.
A systematic approach to quantitative Western blot analysis
Pillai-Kastoori, Schutz-Geschwender, Harford
Analytical Biochemistry (2020) 593, 113608 DOI: 10.1016/j.ab.2020.113608
Cited by 426
AI Summary: OriGene products, including POU5F1 overexpression lysate (LY400950), HEK293T lysate (LY500001), and 2× SDS buffer, were used as standardized protein samples and reagents to ensure consistency and reliability in the quantitative Western blot analysis described in the study.
Snippet: C32 Whole Cell Lysate: sc-2205, POU5F1 overexpression lysate (Origene # LY400950;Lot #O1AF4D);HEK293T lysate (Origene #LY500001 Lot #O1AF4D);were mixed with either 2× protein loading buffer (PLB) (LICOR# 928-40004) OR 2× SDS buffer (OriGene) and denatured by boiling at 97 °C for 5 min. The aliquots were then immediately placed on ice for 5 min before being spun down
Treatment with the cancer drugs decitabine and doxorubicin induces human skin keratinocytes to express Oct4 and the OCT4 regulator mir-145
Chinnathambi, Wiechert, Tomanek-Chalkley et al
J. Dermatol. (2012) 39 (7), 617-24 DOI: 10.1111/j.1346-8138.2012.01553.x
Cited by 11
AI Summary: In this study, OriGene's Turbofectin was used to transiently transfect keratinocytes with pMSCV-OCT4, enabling high Oct4 expression and facilitating the investigation of DNA demethylation and gene regulation following treatment with cancer drugs.
Snippet: C in a 5% CO 2 humidified incubator until 60–70% confluent, then transiently transfected with pMSCV-OCT4 using Turbofectin (Origene, Rockville, MD ... Because it was reported that a short treatment with decitabine inhibits DNA methylation in various cell types, 8 we tested this
Showing 1-4 of 4 papers.
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