ME1 (NM_002395) Human Over-expression Lysate
$206.00
2 Weeks*
Specifications
| Product Data | |
| Species | Human |
|---|---|
| Expression Host | HEK293T |
| Expression cDNA Clone or AA Sequence | TrueORF Clone RC206820 |
| Tag | C-Myc/DDK |
| Detection Antibodies | Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100) |
| ACCN | NM_002395, NP_002386 |
| Synonyms | HUMNDME; MES |
| Predicted MW | 64.1 kDa |
| Components | 1 vial of 20 ug lyophilized total protein derived from gene specific transient over-expression cell lysate |
| Storage | The lysate can be shipped at ambient temperature, however upon receipt of the sample store at -20°C. Lysate samples are stable for 12 months from the date of receipt when stored at -20°C in their original lyophilized form. After reconstitution, avoid repeat freeze thaw cycles and store at -80°C. Lysate samples are stable for 12 months from date of receipt when stored at -20°C. |
| Shipping | Blue Ice |
| Preparation | HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins. |
| Reconstitution Method | The 20 µg lyophilized sample can be reconstituted with deionized water, or directly into 1x SDS-PAGE running buffer (with reducing agent) if the sample is being prepared directly to be run on a gel. The lyophilized 20 ug product is derived from a liquid solution at 1 mg/ml of total lysate protein, thus reconstituting back to 1 mg/ml using 20 µl is recommended. After reconstitution, avoid repeat freeze thaw cycles and store at -80°C. |
| Reference Data | |
| RefSeq | NP_002386 |
| Locus ID | 4199 |
| Cytogenetics | 6q14.2 |
| Protein Families | WB |
| Protein Pathways | Metabolic pathways, PPAR signaling pathway, Pyruvate metabolism |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
| Recombinant Protein Resources |
Other "ME1" proteins (3)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| PH306820 | ME1 MS Standard C13 and N15-labeled recombinant protein (NP_002386) | 10 ug |
$3,360.00
|
|
| LY400854 | Transient overexpression lysate of malic enzyme 1, NADP(+)-dependent, cytosolic (ME1) (as WB positive control) | 100 ug |
$436.00
|
|
| TP306820 | Recombinant protein of human malic enzyme 1, NADP(+)-dependent, cytosolic (ME1), 20 µg | 20 ug |
$737.00
|
|
Citations
The HMGB1 protein induces a metabolic type of tumour cell death by blocking aerobic respiration
Gdynia, Sauer, Kopitz et al
Nat Commun (2016) 7, 10764 DOI: 10.1038/ncomms10764
Gdynia, Sauer, Kopitz et al
Nat Commun (2016) 7, 10764 DOI: 10.1038/ncomms10764
Cited by 70
AI Summary: In this study, the OriGene pCMV-ME1 plasmid was used to overexpress malic enzyme 1 (ME1) in cancer cells, enabling the researchers to investigate ME1’s role in metabolic resistance to HMGB1-induced tumour cell death.
Snippet: ME1 knockdown was performed with 40 nM siRNA using lipofectamine in 6-well plates followed by treatment with 80 nM HMGB1 for 72 h. Sequences of siRNA were: ME1, 5′-CCCUGUGGGUAAAUUGGCUCUAUAU-3′ and scrambled control 5′-CCUGCAGUACUUCAAGCGGtt-3′. PKM2 siRNA was from Santa Cruz. … For overexpression of PKM2 or ME1, cells were transfected with pCMV-PKM2 (Sino Biological Inc., Beijing, China) or pCMV-ME1 (OriGene, Rockville, MD, USA) using Lipofectamine 2000. …
Showing 1-1 of 1 papers.
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
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