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GLP1R (NM_002062) Human Over-expression Lysate

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SKU
LC400753
Lyophilized GLP1R HEK293T cell transient overexpression lysate (as WB positive control)
Size:
20 ug 100 ug
Formulation:
Lyophilized Liquid
  $206.00
2 Weeks*
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC211333
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_002062, NP_002053
Synonyms GLP-1; GLP-1-R; GLP-1R
Predicted MW 52.8 kDa
Components 1 vial of 20 ug lyophilized total protein derived from gene specific transient over-expression cell lysate
Storage The lysate can be shipped at ambient temperature, however upon receipt of the sample store at -20°C. Lysate samples are stable for 12 months from the date of receipt when stored at -20°C in their original lyophilized form.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C. Lysate samples are stable for 12 months from date of receipt when stored at -20°C.
Shipping Blue Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution Method The 20 µg lyophilized sample can be reconstituted with deionized water, or directly into 1x SDS-PAGE running buffer (with reducing agent) if the sample is being prepared directly to be run on a gel.  The lyophilized 20 ug product is derived from a liquid solution at 1 mg/ml of total lysate protein, thus reconstituting back to 1 mg/ml using 20 µl is recommended.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C.
Reference Data
RefSeq NP_002053
Locus ID 2740
Cytogenetics 6p21.2
Protein Families WB
Protein Pathways Neuroactive ligand-receptor interaction
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Other "GLP1R" proteins (1)
SKU Description Size Price
LY400753 Transient overexpression lysate of glucagon-like peptide 1 receptor (GLP1R) (as WB positive control) 100 ug
$436.00

file-alt Citations

A proopiomelanocortin-derived peptide sequence enhances plasma stability of peptide drugs
Löw, Roulin, Kunz
FEBS Lett (2020) 594 (17), 2840-2866 DOI: 10.1002/1873-3468.13855
Cited by 6
AI Summary: In this paper, OriGene products—specifically the human POMC cDNA clone (RC216541) and the human POMC shRNA plasmid (TL314880)—were used to facilitate the expression and knockdown of POMC in cellular experiments, enabling the study of plasma-stabilizing properties of POMC-derived peptide sequences.
Glucagon-Like Peptide-1 Receptor Activation in the Ventral Tegmental Area Decreases the Reinforcing Efficacy of Cocaine
Schmidt, Mietlicki-Baase, Ige et al
Neuropsychopharmacology (2016) 41 (7), 1917-28 DOI: 10.1038/npp.2015.362
Cited by 148
AI Summary: OriGene products contributed to this study by providing the AAV-shRNA used for knockdown of GLP-1 receptors in the ventral tegmental area, enabling the authors to demonstrate that reduced GLP-1 receptor expression increases cocaine self-administration.
Quantitative Impact of Plasma Clearance and Down-regulation on GLP-1 Receptor Molecular Imaging
Zhang, Thurber
Mol Imaging Biol (2015)
Cited by 18
AI Summary: OriGene's GLP-1R (GFP-tagged) plasmid was used to generate stable HEK293 cell lines expressing the GLP-1 receptor, enabling in vitro studies of receptor internalization and imaging probe binding in this research.
Snippet: To generate a stable cell line, HEK293 cells were first cultured in six-well plates and transfected using Lipofectamine 2000 following the manufacturer's instructions to express GLP-1R (GFP-tagged, Origene) and then selected with 1 mg/ml G-418. ... Cite SaveSaving
Showing 1-3 of 3 papers.
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