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PARP1 (NM_001618) Human Over-expression Lysate

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SKU
LC400609
Lyophilized PARP1 HEK293T cell transient overexpression lysate (as WB positive control)
Size:
20 ug 100 ug
Formulation:
Lyophilized Liquid
  $206.00
2 Weeks*
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC207085
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_001618, NP_001609
Synonyms ADPRT; ADPRT 1; ADPRT1; ARTD1; pADPRT-1; PARP; PARP-1; PPOL
Predicted MW 112.9 kDa
Components 1 vial of 20 ug lyophilized total protein derived from gene specific transient over-expression cell lysate
Storage The lysate can be shipped at ambient temperature, however upon receipt of the sample store at -20°C. Lysate samples are stable for 12 months from the date of receipt when stored at -20°C in their original lyophilized form.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C. Lysate samples are stable for 12 months from date of receipt when stored at -20°C.
Shipping Blue Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution Method The 20 µg lyophilized sample can be reconstituted with deionized water, or directly into 1x SDS-PAGE running buffer (with reducing agent) if the sample is being prepared directly to be run on a gel.  The lyophilized 20 ug product is derived from a liquid solution at 1 mg/ml of total lysate protein, thus reconstituting back to 1 mg/ml using 20 µl is recommended.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C.
Reference Data
RefSeq NP_001609
Locus ID 142
Cytogenetics 1q42.12
Protein Families WB
Protein Pathways Base excision repair
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Other "PARP1" proteins (4)
SKU Description Size Price
PH307085 PARP1 MS Standard C13 and N15-labeled recombinant protein (NP_001609) 10 ug
$3,360.00
LY400609 Transient overexpression lysate of poly (ADP-ribose) polymerase 1 (PARP1) (as WB positive control) 100 ug
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TP307085 Purified recombinant protein of Homo sapiens poly (ADP-ribose) polymerase 1 (PARP1), 20 µg 20 ug
$737.00
TP710053 Recombinant protein of human poly (ADP-ribose) polymerase 1 (PARP1),full length, with C-terminal flag tag,expressed in sf9 cells 20 ug
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file-alt Citations

RECQ1 Promotes Stress Resistance and DNA Replication Progression Through PARP1 Signaling Pathway in Glioblastoma
Zhang, Lian, Chen et al
Front Cell Dev Biol (2021) 9, 714868 DOI: 10.3389/fcell.2021.714868
Cited by 7
AI Summary: OriGene products, specifically the RECQ1 shRNA (Cat# TL313863) and RECQ1 cDNA plasmid (Cat# RC224706), were used in this study to modulate RECQ1 expression in glioblastoma cells, enabling the authors to investigate the role of RECQ1 in DNA replication stress resistance and its interaction with PARP1 signaling.
FigureFigureFigure
Ubiquitin ligase RNF146 regulates tankyrase and Axin to promote Wnt signaling
Callow, Tran, Phu et al
PLoS ONE (2011) 6 (7), e22595 DOI: 10.1371/journal.pone.0022595
Cited by 228
AI Summary: In this paper, OriGene products including RNF146 cDNA (RC210989), TNKS1 cDNA (RC220275), and TNKS2 cDNA (RC216698) were used to study the roles of RNF146, tankyrase, and Axin in regulating Wnt signaling through protein ubiquitylation and degradation.
FigureFigureFigure
Showing 1-2 of 2 papers.
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