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RUNX2 (NM_001024630) Human Over-expression Lysate

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SKU
LC400402
Lyophilized RUNX2 HEK293T cell transient overexpression lysate (as WB positive control)
Size:
20 ug 100 ug
Formulation:
Lyophilized Liquid
  $206.00
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC212884
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_001024630, NP_001019801
Synonyms AML3; CBF-alpha-1; CBFA1; CCD; CCD1; CLCD; OSF-2; OSF2; PEA2aA; PEBP2aA
Predicted MW 64.44 kDa
Components 1 vial of 20 ug lyophilized total protein derived from gene specific transient over-expression cell lysate
Storage The lysate can be shipped at ambient temperature, however upon receipt of the sample store at -20°C. Lysate samples are stable for 12 months from the date of receipt when stored at -20°C in their original lyophilized form.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C. Lysate samples are stable for 12 months from date of receipt when stored at -20°C.
Shipping Blue Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution Method The 20 µg lyophilized sample can be reconstituted with deionized water, or directly into 1x SDS-PAGE running buffer (with reducing agent) if the sample is being prepared directly to be run on a gel.  The lyophilized 20 ug product is derived from a liquid solution at 1 mg/ml of total lysate protein, thus reconstituting back to 1 mg/ml using 20 µl is recommended.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C.
Reference Data
RefSeq NP_001019801
Locus ID 860
Cytogenetics 6p21.1
Protein Families WB
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Other "RUNX2" proteins (4)
SKU Description Size Price
LC401387 Lyophilized RUNX2 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LY400402 Transient overexpression lysate of runt-related transcription factor 2 (RUNX2), transcript variant 1 (as WB positive control) 100 ug
$665.00
LY401387 Transient overexpression lysate of runt-related transcription factor 2 (RUNX2), transcript variant 3 (as WB positive control) 100 ug
$665.00
TP760214 Recombinant protein of human runt-related transcription factor 2 (RUNX2), transcript variant 3, full length, with N-terminal HIS tag, expressed in E.Coli, 50ug 50 ug
$270.00

file-alt Citations

Runx2 mediated Induction of Novel Targets ST2 and Runx3 Leads to Cooperative Regulation of Hypertrophic Differentiation in ATDC5 Chondrocytes
Bonyadi Rad, Musumeci, Pichler et al
Sci Rep (2017) 7 (1), 17947 DOI: 10.1038/s41598-017-18044-z
AI Summary: OriGene products contributed to this study by providing cDNA clones for murine ST2L (NM_001025602), murine sST2 (NM_010743), murine Runx2 (NM_001145920), and human Runx2 (NM_001024630), which were used in overexpression experiments to investigate the regulation of chondrocyte hypertrophic differentiation.
Snippet: transfection. For overexpression experiments, cDNA clones encoding murine ST2L (NM_001025602), murine sST2 (NM_010743), murine (NM-001145920) and human (NM-001024630) Runx2 were purchased from Origene
FigureFigureFigure
Showing 1-1 of 1 papers.
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