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ALDH3A1 (NM_000691) Human Over-expression Lysate

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SKU
LC400232
Lyophilized ALDH3A1 HEK293T cell transient overexpression lysate (as WB positive control)
Size:
20 ug 100 ug
Formulation:
Lyophilized Liquid
  $206.00
2 Weeks*
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC202440
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_000691, NP_000682
Synonyms ALDH3; ALDHIII
Predicted MW 50.4 kDa
Components 1 vial of 20 ug lyophilized total protein derived from gene specific transient over-expression cell lysate
Storage The lysate can be shipped at ambient temperature, however upon receipt of the sample store at -20°C. Lysate samples are stable for 12 months from the date of receipt when stored at -20°C in their original lyophilized form.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C. Lysate samples are stable for 12 months from date of receipt when stored at -20°C.
Shipping Blue Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution Method The 20 µg lyophilized sample can be reconstituted with deionized water, or directly into 1x SDS-PAGE running buffer (with reducing agent) if the sample is being prepared directly to be run on a gel.  The lyophilized 20 ug product is derived from a liquid solution at 1 mg/ml of total lysate protein, thus reconstituting back to 1 mg/ml using 20 µl is recommended.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C.
Reference Data
RefSeq NP_000682
Locus ID 218
Cytogenetics 17p11.2
Protein Families WB
Protein Pathways Drug metabolism - cytochrome P450, Glycolysis / Gluconeogenesis, Histidine metabolism, Metabolic pathways, Metabolism of xenobiotics by cytochrome P450, Phenylalanine metabolism, Tyrosine metabolism
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Other "ALDH3A1" proteins (11)
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PH325751 ALDH3A1 MS Standard C13 and N15-labeled recombinant protein (NP_001128640) 10 ug
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LC427569 Lyophilized ALDH3A1 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
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LC427570 Lyophilized ALDH3A1 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
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LY400232 Transient overexpression lysate of aldehyde dehydrogenase 3 family, memberA1 (ALDH3A1), transcript variant 2 (as WB positive control) 100 ug
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LY427569 Transient overexpression lysate of aldehyde dehydrogenase 3 family, memberA1 (ALDH3A1), transcript variant 3 (as WB positive control) 100 ug
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LY427570 Transient overexpression lysate of aldehyde dehydrogenase 3 family, memberA1 (ALDH3A1), transcript variant 1 (as WB positive control) 100 ug
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TP302440 Recombinant protein of human aldehyde dehydrogenase 3 family, memberA1 (ALDH3A1), transcript variant 2, 20 µg 20 ug
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TP325750 Recombinant protein of human aldehyde dehydrogenase 3 family, memberA1 (ALDH3A1), transcript variant 3, 20 µg 20 ug
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TP325751 Recombinant protein of human aldehyde dehydrogenase 3 family, memberA1 (ALDH3A1), transcript variant 1, 20 µg 20 ug
$737.00

file-alt Citations

Mutation of the gene encoding the circadian clock component PERIOD2 in oncogenic cells confers chemoresistance by up-regulating the Aldh3a1 gene
Katamune, Koyanagi, Hashikawa et al
J. Biol. Chem. (2018) 294 (2), 547-558 DOI: 10.1074/jbc.RA118.004942
AI Summary: OriGene's pGFP-C-shAldh3a1 Lenti Vector was used to deliver shRNA targeting the mouse Aldh3a1 gene, enabling the researchers to down-regulate ALDH3A1 expression and assess its role in chemoresistance in oncogenic cells.
Snippet: To down-regulate ALDH3A1 expression, cells were infected with lentiviral vectors expressing shRNA against the mouse Aldh3a1 gene (pGFP-C-shAldh3a1 Lenti Vector;Origene Technologies, Inc., Rockville, MD). After infection, cells were maintained in DMEM
Showing 1-1 of 1 papers.
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