TPP1 (NM_000391) Human Over-expression Lysate
$206.00
In Stock*
Specifications
| Product Data | |
| Species | Human |
|---|---|
| Expression Host | HEK293T |
| Expression cDNA Clone or AA Sequence | TrueORF Clone RC204471 |
| Tag | C-Myc/DDK |
| Detection Antibodies | Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100) |
| ACCN | NM_000391, NP_000382 |
| Synonyms | CLN2; GIG1; LPIC; SCAR7; TPP-1 |
| Predicted MW | 61.2 kDa |
| Components | 1 vial of 20 ug lyophilized total protein derived from gene specific transient over-expression cell lysate |
| Storage | The lysate can be shipped at ambient temperature, however upon receipt of the sample store at -20°C. Lysate samples are stable for 12 months from the date of receipt when stored at -20°C in their original lyophilized form. After reconstitution, avoid repeat freeze thaw cycles and store at -80°C. Lysate samples are stable for 12 months from date of receipt when stored at -20°C. |
| Shipping | Blue Ice |
| Preparation | HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins. |
| Reconstitution Method | The 20 µg lyophilized sample can be reconstituted with deionized water, or directly into 1x SDS-PAGE running buffer (with reducing agent) if the sample is being prepared directly to be run on a gel. The lyophilized 20 ug product is derived from a liquid solution at 1 mg/ml of total lysate protein, thus reconstituting back to 1 mg/ml using 20 µl is recommended. After reconstitution, avoid repeat freeze thaw cycles and store at -80°C. |
| Reference Data | |
| RefSeq | NP_000382 |
| Locus ID | 1200 |
| Cytogenetics | 11p15.4 |
| Protein Families | WB |
| Protein Pathways | Lysosome |
Reviews
Documents
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Resources
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Citations
Mitoribosomal Deregulation Drives Senescence via TPP1-Mediated Telomere Deprotection
Min, Kwon, Hong et al
Cells (2022) 11 (13) DOI: 10.3390/cells11132079
Min, Kwon, Hong et al
Cells (2022) 11 (13) DOI: 10.3390/cells11132079
Cited by 5
AI Summary: In this study, the TPP1 overexpression plasmid (pCMV6-ACD-AC-GFP) from OriGene was used to investigate the role of TPP1 in mitoribosomal deregulation and its impact on cellular senescence.
Snippet: UACCACAGGAA), POT1 (#1, 5′-CUGAUGUGGAUCAACUGAA;#2, 5′-CUGAUCAAGCUACAGUGUA), and negative control (5′-CCUACGCCACCAAUUUGGU). The TPP1 overexpression plasmid (pCMV6-ACD-AC-GFP) was purchased from OriGene Technologies (Rockville, MD, USA) and introduced into HDFs using Fugene HD Reagent (Promega, Fitchburg, WI, USA). 2.10. Western Blot Analysis Cells were washed twice with PBS and lysed in R
Showing 1-1 of 1 papers.
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