UBE2H (NM_182697) Human Mass Spec Standard

CAT#: PH313095

UBE2H MS Standard C13 and N15-labeled recombinant protein (NP_874356)

Product Images

Coomassie blue staining of purified UBE2H protein (Cat# TP313095). The protein was produced from HEK293T cells transfected with UBE2H cDNA clone (Cat# RC213095) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Tag: C-Myc/DDK
• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence: RC213095
• Predicted MW: 17 kDa
• Protein Sequence:
  Sequence Link

  >RC213095 representing NM_182697
  Red=Cloning site Green=Tags(s)
  
  MSSPSPGKRRMDTDVVKLIESKHEVTILGGLNEFVVKFYGPQGTPYEGGVWKVRVDLPDKYPFKSPSIDL
  TNIFESFLPQLLAYPNPIDPLNGDAAAMYLHRPEEYKQKIKEYIQKYATEEALKEQEEGTGDSSSESSMS
  DFSEDEAQDMEL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: >0.05 µg/µL as determined by microplate BCA method
• Labeling Method: Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
• Buffer: 25 mM Tris-HCl, 100 mM glycine, pH 7.3
• Storage: Store at -80°C. Avoid repeated freeze-thaw cycles.
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.

Reference Data

• RefSeq: NP_874356
• RefSeq Size: 2635
• RefSeq ORF: 456
• Synonyms: E2-20K; GID3; UBC8; UBCH; UBCH2
• Locus ID: 7328
• UniProt ID: P62256
• Cytogenetics: 7q32.2
• Summary: The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein sequence is 100% identical to the mouse homolog and 98% identical to the frog and zebrafish homologs. Three alternatively spliced transcript variants have been found for this gene and they encode distinct isoforms. [provided by RefSeq, Feb 2011]
• Protein Pathways: Ubiquitin mediated proteolysis