E2F1 (NM_005225) Human Mass Spec Standard

CAT#: PH308247

E2F1 MS Standard C13 and N15-labeled recombinant protein (NP_005216)

Bulk Request/Custom Protein Development

USD 1,995.00


Availability*
3 Weeks

Size
    • 10 ug

Product images

Specifications

Product Data
Description E2F1 MS Standard C13 and N15-labeled recombinant protein (NP_005216)
Species Human
Expression Host HEK293
Expression cDNA Clone or AA Sequence RC208247
Predicted MW 46.7 kDa
Protein Sequence
Tag C-MYC/DDK
Purity > 80% as determined by SDS-PAGE and Coomassie blue staining
Concentration 50 ug/ml as determined by BCA
Labeling Method Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
Buffer 100 mM glycine, 25 mM Tris-HCl, pH 7.3. Store at -80C. Avoid repeated freeze-thaw cycles. Stable for 3 months from receipt of products under proper storage and handling conditions.
Reference Data
RefSeq NP_005216
RefSeq Size 2486
RefSeq ORF 1311
Synonyms E2F-1; RBAP1; RBBP3; RBP3
Locus ID 1869
Cytogenetics 20q11.22
Summary The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F2 and E2F3, have an additional cyclin binding domain. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent/independent apoptosis. [provided by RefSeq, Jul 2008]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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