Gapdh Mouse qPCR Template Standard (NM_008084)

Snippet: ing onto nitrocellulose membranes as is described in detail [5, 9–11]. Detection of proteins on membranes was performed with exposure to primary antibodies as follows, for FCGR1A (mouse monoclonal, Origene, Rockville, MD, USA), FCGR1B (rabbit polyclonal, ThermoFischer, Rockfort, IL or Invitrogen, Waltham, MA, USA), GBP1 (rabbit polyclonal, Proteintech, Rosemont, IL, USA), GCH1 (mouse monoclonal,

Snippet: n;Cell Signaling Technology, Inc.), caspase-9 (mouse, 1:1000 dilution;Santa Cruz Biotechnology), caspase-3 (rabbit, 1:1000 dilution;Cell Signaling Technology, Inc.), GAPDH (mouse, 1:1000 dilution;OriGene, Beijing, China), PCNA (mouse, 1:1000 dilution;OriGene) and β-tubulin (mouse, 1:1000 dilution;OriGene). The secondary antibodies used in this study were goat anti-mouse IgG (peroxidase conj

Snippet: Technology, Inc), phosho‐IκBα (rabbit, 1:1000 dilution, Cell Signaling Technology, Inc), IκBα (rabbit, 1:1000 dilution, Cell Signaling Technology, Inc), NF‐κB (p65;rabbit, 1:1000 dilution, Cell Signaling Technology, Inc), PCNA (mouse, 1:1000 dilu‐ tion, OriGene), β‐tubulin

Snippet: For validation experiments in HEK293T (cultured in DMEM, 10% FBS, 2 mM LGlutamine, 37o C, 5% CO2) cells were transfected with plasmids expressing C-terminal c-Myc tagged SEC11A (Origene) and C-terminal FLAG-tagged mouse NNAT in pcDNA3.1 (BamHI/XhoI sites) using Lipofectamine 2000. 48 hours later, transfected cells were lysed, clarified, normalized as above and immunoprecipitated using anti-cMyc agarose resin (Pierce) with Western blotting performed using antibodies against c-Myc (mouse monoclonal, Cell Signalling) and FLAG (mouse monoclonal, SigmaAldrich)

Snippet: epithelial cells (16). The pathological relevance of this in vitro observationwas investigated by measuring mRNA levels of S1PR3 in cDNA microarrays of human lungadenocarcinoma specimens (Origene, HLRT). Quantitative PCR ... Cite SaveSaving