U6 Human qPCR Primer Pair

SKU
MP300001
qSTAR human and mouse U6 control miRNA primer pairs--200 reactions
  $150.00
2 Weeks*
Specifications
Specifications
Product Data
Forward Sequence CTCGCTTCGGCAGCACAT
Reverse Sequence TTTGCGTGTCATCCTTGCG
Synonyms U6
Components 1 vial of lyophilized qSTAR miRNA primer mix (1 nmol each primer, sufficient for 200 reactions)
Quality Control The primer mix has been tested to generate satisfactory qPCR data on ABI 7900HT by using the following PCR program: Stage 1: Activation: 50 °C for 2 min; Stage 2: pre-soak:95 °C for 10 min; Stage 3: Denaturation: 95 °C for 15 sec, Annealing: 60°C for 1 min; Stage 4: Melting curve: 95°C for 15 sec, 60°C for 15 sec, 95°C for 15 sec.
Storage The primer mix is stable for one year from date of shipping. Store at -20°C.
Shipping Blue Ice
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Documents
Resources

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Downregulation of miR-221, miR-143, and miR-22 in Meningioma: Diagnostic Performance in a Single-Center Case-Control Study
Tekir, Ozkara, Erzurumluoglu et al
Turk Neurosurg (2026) 36 (3), 371-376 DOI: 10.5137/1019-5149.JTN.49747-25.3
AI Summary: OriGene products—specifically miR-143 (#MI0000461), miR-221 (#MI0000298), miR-22 (#MI0000078), and U6 (#MP300001)—were used as qRT-PCR assays and normalization controls to measure and compare miRNA expression levels in meningioma and control tissues in this study.
Snippet: 461), miR-221 (#MI0000298), and miR-22 (#MI0000078) were obtained from OriGene Technologies (Beijing, China). Gene expression levels were normalized to U6 (#MP300001), and relative expression was calculated using the 2−ΔΔCt method (13). Statistical Analysis Statistical analysis was perfor
Patients with abdominal aortic aneurysms have reduced levels of microRNA 122-5p in circulating exosomes
Lopez, Ramirez, Phu et al
PLoS One (2023) 18 (2), e0281371 DOI: 10.1371/journal.pone.0281371
Cited by 6
AI Summary: OriGene contributed to this study by providing the Human qPCR Primer Pair (Catalog number: HP300285) and U6 Human qPCR Primer Pair (Catalog number: MP300001), which were used for validating microRNA expression profiles by quantitative real-time PCR.
Snippet: Human qPCR Primer Pair Catalog number: HP300285, U6 Human qPCR Primer Pair Catalog number: MP300001) were all purchased from and designed by OriGene
FigureFigureFigure
Downregulation of miR-126-3p expression contributes to increased inflammatory response in placental trophoblasts in preeclampsia
Chu, Gu, Sheng et al
J Reprod Immunol (2021) 144, 103281 DOI: 10.1016/j.jri.2021.103281
Cited by 34
AI Summary: OriGene’s pre-mir-126 (a precursor of miR-126) was used to transiently overexpress miR-126-3p in primary trophoblasts, enabling the authors to demonstrate that increased miR-126-3p expression suppresses inflammatory cytokine production and oxidative stress in placental cells relevant to preeclampsia.
FoxF1 protects rats from paraquat-evoked lung injury following HDAC2 inhibition via the microRNA-342/KLF5/IκB/NF-κB p65 axis
Zheng, Liu, Zhu et al
Exp Cell Res (2020) 395 (2), 112208 DOI: 10.1016/j.yexcr.2020.112208
Cited by 18
AI Summary: In this study, OriGene products including the FoxF1 expression plasmid (RC210945) and FoxF1 siRNA (SR506113) were used to modulate FoxF1 levels in cell experiments, enabling the authors to investigate the role of FoxF1 in paraquat-induced lung injury and its underlying molecular mechanisms.
Long Noncoding RNA KCNQ1OT1 Confers Gliomas Resistance to Temozolomide and Enhances Cell Growth by Retrieving PIM1 From miR-761
Wang, Han, Gao et al
Cell. Mol. Neurobiol. (2020)
Cited by 21
AI Summary: OriGene contributed to this study by synthesizing the primers (including those for GAPDH and U6) used in gene expression analyses.
Snippet: 3′. GAPDH, forward, 5′-GTCTCCTCTGACTTCAACAGCG-3′;reverse, 5′-ACCACCCTGTTGCTGTAGCCAA-3′. U6, forward, 5′-CTCGCTTCGGCAGCACAT-3′;reverse, 5′-TTTGCGTGTCATCCTTGCG-3′. All primers were synthesized in OriGene (Rockville
Sevoflurane attenuates cardiomyocyte apoptosis by mediating the miR-219a/AIM2/TLR4/MyD88 axis in myocardial ischemia/reperfusion injury in mice
Li, Xing, Yuan et al
Cell Cycle (2020) 19 (13), 1665-1676 DOI: 10.1080/15384101.2020.1765512
Cited by 28
AI Summary: OriGene products, specifically the primer sequences for miR-219a (#RP300127), U6 (#MP3001), AIM2 (#MP200186), and GAPDH (#MP205604), were used for RT-qPCR analysis to measure gene expression levels in this study investigating the protective effects of sevoflurane on myocardial ischemia/reperfusion injury.
Snippet: Primer sequences of miR-219a (#RP300127), U6 (#MP3001), AIM2 (#MP200186), and GAPDH (#MP205604) were purchased from OriGene Technologies (Beijing, China). 2.8. … Starbase;- Primer sequences of miR-219a (#RP300127), U6 (#MP3001), AIM2 (#MP200186), and GAPDH (#MP205604) were purchased from OriGene Technologies (Beijing, China). 2.8. …
Showing 1-6 of 12 papers.
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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