IL6 Human qPCR Primer Pair (NM_000600)

Snippet: triplicates using Power Track SYBR Green Mastermix (Applied Biosystems;Waltham, MA, USA, A46109) on the QuantStudio 3 Real-Time PCR System. qSTAR qPCR primers were purchased from OriGene (Rockville, MD, USA): IL-6: HP200567;IL-8 (CXCL8): HP200551;IL-1β: HP200544;GAPDH: HP205798. Relative mRNA expression was determined using the ΔΔCt method

Snippet: , #HP200567) gene expression and IL- 8 (Origene, #HP200551), human qPCR primer pairs were obtained from Origene. GAPDH was used as a housekeeping gene

Snippet: The cDNA from various cell samples was then amplified by real-time quantitative polymerase chain reaction (qPCR) with predesigned primers (TNF: #HP200561;IL6: #HP200567;IFNA1: #HP214678) from OriGene using an CFX96 Touch Real-Time PCR Detection;- The cDNA from various cell samples was then amplified by real-time quantitative polymerase chain reaction (qPCR) with predesigned primers (TNF: #HP200561

Snippet: expression. The primers of IL‐6, IL‐8, and GAPDH were previously described and the primers of CBP, p300 as well as IL‐10 were from the available qPCR Primer Pairs of OriGene Technologies (Rockville, MD) (Table 1).8. Table

Snippet: instructions. Validated qPCR primers were purchased from Qiagen or OriGene, and available primer sequences are provided in Table 1. Approximately 3 or 6 ng of polyA-mRNA (isolated previously) was mixed with 5 µL of 1 ... Cite SaveSaving