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HGD Human Gene Knockout Kit (CRISPR)

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SKU
KN210009BN
HGD - human gene knockout kit via CRISPR, HDR mediated
  $1,657.00
4 Weeks*

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Specifications
Specifications
Product Data
Format 2 gRNA vectors, 1 mBFP-Neo donor, 1 scramble control
Vector mBFP-Neo
Target Symbol HGD
Locus ID 3081
Components

KN210009G1, HGD gRNA vector 1 in pCas-Guide CRISPR vector

KN210009G2, HGD gRNA vector 2 in pCas-Guide CRISPR vector

KN210009BND, donor DNA containing left and right homologous arms and mBFP-Neo functional cassette.

Homologous arm and mBFP-Neo sequences:

pUC vector backbone in gray; Left arm sequence in blue; mBFP-Neo in green; Right arm in violet

GAACCTGAGC TCATTCCTGG GAGGCACCTG CAGAAAACTG GTTCTGGTCC TGGTCCCTGT CTTCAGGTGG CTGTGTGTAA ACATCTTACC CCAGTGTACA CATCTGCAGC TGGTAAATGC CTGGGGGTTT TCTCACTGCT CTCCAGGAGA CTCAGCACTG CCCTGTTCCT TCCTAGCCTG AGTCAGGACT GAGCAGAGAA TCCTCCACCA CACACAGCAG GCACTATCTG CCACAGTTCC TTTCCCCGAT AGCTTCAAAT TCTCTGCCTT TTGAAATAAG CCTACTTTTA ACTGGAATAA ATAATTGGTC AATCTCTACC TCAGGTGAAG AGGAACCAAG CCTCTGGAAA CACTTAGGAA CAAACTGTAA AAACCAAAGG CAATTGTGTA ACCGGTTAAA TAAGCTTGCT GGACTTTGTC CCTGTGTATG AGTTAGACAA TTCTTTCAGC TAGTTTGAGT GACGCACTGA CCAGTGAAGC GCAGTGAAGC AGTGGGAACC GGAATATCCA AAGAGTGGTT TGAAGGAGAA AGAAGCATTG TGGCTTTATA TCCTCTGGGC CTGGGTTTCC TGAAGTCACC ACACATAGAG GAGAGAGAAA GAAAAGACTT AGAGAGGCCC TGGTTCAAAC CCCTGTGTTC AGAGGCTTCC CTGTGGTGTC TGTGGGCACA GCTGGAGCTG AAGAACAAGG GAAAGGTGAT AAAGGAAAGA AATGAAGCTC TTATTGGTCA TCCCTTGCCT TTGTTCATTT CAATTATTGT CTTGGAAATC TGTAGACATC CTAAGAGCTC CCCTCCACTA CGGACATGCA CGTTTCTCTC TATGTCTTTT TCCCAACCCC TGCTCTGACA CCTTCCCATT GCTGCAGCCT CATGGACCAC TTCCGGTGAC CAGTTAGATA TGCTTGATCT ATTTAGTTAA TTAAGGCTCT GAATCAGCCC TTGGCCAGAA GAAGCACCTC CTCTAGGAAA TCATTCCTTC TTTTTAGTAA TACGCAATTG AAAAGTATGT GTGGGTTATG TGCTGTTAGG TGCTAAGCAT TTTAGCTATG AATTAACTCA TAATCCTCAA AATAAATCTG GGGTATAGAG AGAGGTTCTT GTTTCAATTA GAAAAGTAAT GCTTGGAGAG ACTATGATTT GCCCAAGGTC ACTGCCAATA GATGACTTGA TTAGAAGCAG GGTATGTTTG AATGCGAAGT

GE100003, scramble sequence in pCas-Guide vector
OTI Disclaimer These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.
Reference Data
RefSeq NM_000187
UniProt ID Q93099
Synonyms AKU; HGO
Summary This gene encodes the enzyme homogentisate 1,2 dioxygenase. This enzyme is involved in the catabolism of the amino acids tyrosine and phenylalanine. Mutations in this gene are the cause of the autosomal recessive metabolism disorder alkaptonuria.provided by RefSeq, May 2010
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Documents
Resources
Other Versions
SKU Description Size Price
GA102101 HGD CRISPRa kit - CRISPR gene activation of human homogentisate 1,2-dioxygenase 1 kit
$1,657.00
KN210009 HGD - human gene knockout kit via CRISPR, HDR mediated 1 kit
$1,657.00
KN210009LP HGD - human gene knockout kit via CRISPR, HDR mediated 1 kit
$1,657.00
KN210009RB HGD - human gene knockout kit via CRISPR, HDR mediated 1 kit
$1,657.00
KN410009 HGD - KN2.0, Human gene knockout kit via CRISPR, non-homology mediated. 1 kit
$1,657.00

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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