Mouse Rfx6 activation kit by CRISPRa

SKU GA216599

Rfx6 CRISPRa kit - CRISPR gene activation of mouse regulatory factor X, 6

Product Data

• Format: 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug)
• Target Symbol: Rfx6
• Locus ID: 320995
• Components:

  GA216599G1, Rfx6 gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA216599G2, Rfx6 gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA216599G3, Rfx6 gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• OTI Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPRa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001159389, NM_177306
• UniProt ID: Q8C7R7
• Synonyms: 4930572O07Rik; Rfxdc1
• Summary: Transcription factor required to direct islet cell differentiation during endocrine pancreas development. Specifically required for the differentiation of 4 of the 5 islet cell types and for the production of insulin. Not required for pancreatic PP (polypeptide-producing) cells differentiation. Acts downstream of NEUROG3 and regulates the transcription factors involved in beta-cell maturation and function, thereby restricting the expression of the beta-cell differentiation and specification genes, and thus the beta-cell fate choice. Activates transcription by forming a heterodimer with RFX3 and binding to the X-box in the promoter of target genes (PubMed:20148032). Involved in glucose-stimulated insulin secretion by promoting insulin and L-type calcium channel gene transcription (By similarity).[UniProtKB/Swiss-Prot Function]