Mouse Magel2 activation kit by CRISPRa

SKU GA205142

Magel2 CRISPRa kit - CRISPR gene activation of mouse melanoma antigen, family L, 2

Product Data

• Format: 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug)
• Target Symbol: Magel2
• Locus ID: 27385
• Components:

  GA205142G1, Magel2 gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA205142G2, Magel2 gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA205142G3, Magel2 gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• OTI Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPRa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.
• Shipping: Ambient

Reference Data

• RefSeq: NM_013779
• UniProt ID: Q9QZ04
• Synonyms: Mage-l2; NDNL1; nM15; ns7
• Summary: Probably enhances ubiquitin ligase activity of RING-type zinc finger-containing E3 ubiquitin-protein ligases, possibly through recruitment and/or stabilization of the Ubl-conjugating enzyme (E2) at the E3:substrate complex. Acts as a regulator of retrograde transport via its interaction with VPS35. Recruited to retromer-containing endosomes and promotes the formation of 'Lys-63'-linked polyubiquitin chains at 'Lys-220' of WASHC1 together with TRIM27, leading to promote endosomal F-actin assembly (By similarity). Regulates the circadian clock by repressing the transcriptional activator activity of the CLOCK-ARNTL/BMAL1 heterodimer. Significantly promotes the cytoplasmic accumulation of CLOCK (PubMed:22208286).[UniProtKB/Swiss-Prot Function]