Mouse Rfx2 activation kit by CRISPRa

SKU GA203626

Rfx2 CRISPRa kit - CRISPR gene activation of mouse regulatory factor X, 2 (influences HLA class II expression)

Product Data

• Format: 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug)
• Target Symbol: Rfx2
• Locus ID: 19725
• Components:

  GA203626G1, Rfx2 gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA203626G2, Rfx2 gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA203626G3, Rfx2 gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• OTI Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPRa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.
• Shipping: Ambient

Reference Data

• RefSeq: NM_009056, NM_027787
• UniProt ID: P48379
• Synonyms: 5430432H19Rik
• Summary: Transcription factor that acts as a key regulator of spermatogenesis (PubMed:26248850, PubMed:26162102, PubMed:26853561). Acts by regulating expression of genes required for the haploid phase during spermiogenesis, such as genes required for cilium assembly and function (PubMed:26162102, PubMed:26853561). Recognizes and binds the X-box, a regulatory motif with DNA sequence 5'-GTNRCC(0-3N)RGYAAC-3' present on promoters (PubMed:15229132, PubMed:26162102). Probably activates transcription of the testis-specific histone gene HIST1H1T (PubMed:15229132).[UniProtKB/Swiss-Prot Function]