Human DENND1B activation kit by CRISPRa

SKU GA116090

DENND1B CRISPRa kit - CRISPR gene activation of human DENN domain containing 1B

Product Data

• Format: 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug)
• Target Symbol: DENND1B
• Locus ID: 163486
• Components:

  GA116090G1, DENND1B gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA116090G2, DENND1B gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA116090G3, DENND1B gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• OTI Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPRa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001142795, NM_001195215, NM_001195216, NM_001300858, NM_144977, NR_125340
• UniProt ID: Q6P3S1
• Synonyms: C1orf218; DENN/MADD domain containing 1B; DKFZp547O0715; FAM31B; family with sequence similarity 31, member B; FLJ20054; FLJ21179; FLJ33888; MGC27044; MGC103810; RP11-53I24.2
• Summary: Clathrin (see MIM 118955)-mediated endocytosis is a major mechanism for internalization of proteins and lipids. Members of the connecdenn family, such as DENND1B, function as guanine nucleotide exchange factors (GEFs) for the early endosomal small GTPase RAB35 (MIM 604199) and bind to clathrin and clathrin adaptor protein-2 (AP2; see MIM 601024). Thus, connecdenns link RAB35 activation with the clathrin machinery (Marat and McPherson, 2010 [PubMed 20154091]).[supplied by OMIM, Nov 2010]