Human NPL4 (NPLOC4) activation kit by CRISPRa

SKU GA110868

NPLOC4 CRISPRa kit - CRISPR gene activation of human NPL4 homolog, ubiquitin recognition factor

Product Data

• Format: 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug)
• Target Symbol: NPLOC4
• Locus ID: 55666
• Components:

  GA110868G1, NPL4 gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA110868G2, NPL4 gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA110868G3, NPL4 gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• OTI Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPRa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.
• Shipping: Ambient

Reference Data

• RefSeq: NM_017921, NR_130139, NR_130140, NM_001369698
• UniProt ID: Q8TAT6
• Synonyms: NPL4
• Summary: The ternary complex containing UFD1, VCP and NPLOC4 binds ubiquitinated proteins and is necessary for the export of misfolded proteins from the ER to the cytoplasm, where they are degraded by the proteasome. The NPLOC4-UFD1-VCP complex regulates spindle disassembly at the end of mitosis and is necessary for the formation of a closed nuclear envelope (By similarity). Acts as a negative regulator of type I interferon production via the complex formed with VCP and UFD1, which binds to DDX58/RIG-I and recruits RNF125 to promote ubiquitination and degradation of DDX58/RIG-I (PubMed:26471729).[UniProtKB/Swiss-Prot Function]