Human SMG7 activation kit by CRISPRa

SKU GA106675

SMG7 CRISPRa kit - CRISPR gene activation of human SMG7 nonsense mediated mRNA decay factor

Product Data

• Format: 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug)
• Target Symbol: SMG7
• Locus ID: 9887
• Components:

  GA106675G1, SMG7 gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA106675G2, SMG7 gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA106675G3, SMG7 gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• OTI Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPRa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001174061, NM_014837, NM_173156, NM_201568, NM_201569, NM_001331007, NM_001350219, NM_001350220, NM_001350221, NM_001350222
• UniProt ID: Q92540
• Synonyms: C1orf16; EST1C; SGA56M
• Summary: This gene encodes a protein that is essential for nonsense-mediated mRNA decay (NMD); a process whereby transcripts with premature termination codons are targeted for rapid degradation by a mRNA decay complex. The mRNA decay complex consists, in part, of this protein along with proteins SMG5 and UPF1. The N-terminal domain of this protein is thought to mediate its association with SMG5 or UPF1 while the C-terminal domain interacts with the mRNA decay complex. This protein may therefore couple changes in UPF1 phosphorylation state to the degradation of NMD-candidate transcripts. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Aug 2011]