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NB (NC_002209) Virus Tagged ORF Clone

  • TrueORF®
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SKU
VC102484
Myc-DDK-tagged ORF clone of viral ORF for NB protein [Influenza B virus], codon optimized for human cell expression, NP_056662
  $165.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Virus Tagged ORF Clone
Target Symbol NB
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>The Viral ORF clone VC102484 represents NCBI reference of NP_056662 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s)

GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAACAACGCAACTTTTAATTGCACCAACATTAACCCTATCACTCATATACGAGGGTCCATCATCATTA
CTATCTGCGTGTCCCTCATTGTTATCCTCATAGTTTTTGGATGTATCGCCAAGATATTTATCAATAAGAA
CAATTGTACTAACAACGTCATCCGGGTCCACAAAAGGATTAAATGCCCCGATTGTGAGCCTTTCTGTAAC
AAGCGCGATGATATTAGCACTCCCAGAGCAGGGGTGGATATTCCAAGCTTCATCCTTCCAGGCTTGAACC
TGAGTGAAGGGACGCCCAAT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>VC102484 representing NP_056662
Red=Cloning sites Green=Tags

MNNATFNCTNINPITHIRGSIIITICVSLIVILIVFGCIAKIFINKNNCTNNVIRVHKRIKCPDCEPFCN
KRDDISTPRAGVDIPSFILPGLNLSEGTPN

TRTRRLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene |
ACCN NC_002209
ORF Size 300 bp
OTI Disclaimer The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NC_002209.1, NP_056662
RefSeq ORF 300 bp
Locus ID 956540
MW 11.0 kDa
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Documents
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"NC_002209" in other vectors (1)
SKU Description Size Price
VC102485 Myc-DDK-tagged ORF clone of viral ORF for neuraminidase [Influenza B virus], codon optimized for human cell expression, NP_056663 10 ug
$686.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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