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HA1 (NC_002017) Virus Tagged ORF Clone

  • TrueORF®
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SKU
VC102476
Myc-DDK-tagged ORF clone of viral ORF for HA1 [Influenza A virus (A/Puerto Rico/8/1934(H1N1))], codon optimized for human cell expression, YP_163735
  $450.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Virus Tagged ORF Clone
Target Symbol HA1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>The Viral ORF clone VC102476 represents NCBI reference of YP_163735 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s)

GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGACACCATTTGCATCGGATACCACGCTAACAATAGCACCGATACCGTGGACACCGTGCTGGAGAAAA
ACGTGACCGTGACACACAGCGTCAATCTCCTCGAAGACTCCCATAACGGCAAGCTCTGTCGGCTGAAAGG
CATCGCCCCACTGCAGCTGGGAAAATGCAACATCGCCGGCTGGCTGCTCGGCAACCCCGAGTGCGACCCC
CTGCTGCCCGTCCGGTCCTGGAGCTACATCGTCGAGACCCCCAACAGCGAGAACGGCATCTGCTACCCCG
GCGACTTCATCGACTACGAGGAGCTGCGGGAGCAGCTCAGCAGCGTGAGCAGCTTCGAGCGGTTCGAGAT
CTTCCCCAAGGAGAGCAGCTGGCCCAACCACAACACAACCAAGGGCGTGACCGCCGCCTGCTCCCACGCC
GGCAAGAGCAGCTTCTACCGGAACCTGCTGTGGCTGACCGAGAAGGAGGGCAGCTATCCCAAGCTCAAAA
ACAGCTACGTGAACAAGAAGGGCAAGGAGGTCCTGGTCCTGTGGGGCATCCACCACCCCAGCAACAGCAA
GGACCAGCAGAACATCTACCAAAACGAGAACGCCTACGTGTCCGTGGTGACTAGCAATTACAACCGGCGG
TTCACCCCCGAGATCGCCGAGCGGCCCAAAGTGCGGGACCAGGCAGGCCGGATGAACTACTACTGGACTC
TGCTCAAGCCCGGCGACACCATCATCTTCGAGGCCAACGGGAACCTGATCGCCCCCCGGTACGCCTTTGC
CCTGAGCCGGGGCTTCGGCAGCGGCATCATCACCTCCAACGCCAGCATGCACGAATGCAACACCAAGTGC
CAGACCCCCCTGGGCGCAATCAACAGCAGCCTGCCCTTCCAGAATATCCACCCTGTGACTATCGGCGAGT
GCCCCAAGTACGTGCGGAGCGCTAAGCTGAGAATGGTCACTGGCCTGCGGAATATTCCATCCATCCAGAG
CCGG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>VC102476 representing YP_163735
Red=Cloning sites Green=Tags

MDTICIGYHANNSTDTVDTVLEKNVTVTHSVNLLEDSHNGKLCRLKGIAPLQLGKCNIAGWLLGNPECDP
LLPVRSWSYIVETPNSENGICYPGDFIDYEELREQLSSVSSFERFEIFPKESSWPNHNTTKGVTAACSHA
GKSSFYRNLLWLTEKEGSYPKLKNSYVNKKGKEVLVLWGIHHPSNSKDQQNIYQNENAYVSVVTSNYNRR
FTPEIAERPKVRDQAGRMNYYWTLLKPGDTIIFEANGNLIAPRYAFALSRGFGSGIITSNASMHECNTKC
QTPLGAINSSLPFQNIHPVTIGECPKYVRSAKLRMVTGLRNIPSIQSR

TRTRRLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene |
ACCN NC_002017
ORF Size 984 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NC_002017.1, YP_163735
RefSeq ORF 984 bp
MW 36.8 kDa
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"NC_002017" in other vectors (2)
SKU Description Size Price
VC102475 Myc-DDK-tagged ORF clone of viral ORF for haemagglutinin [Influenza A virus (A/Puerto Rico/8/1934(H1N1))], codon optimized for human cell expression, NP_040980 10 ug
$790.00
VC102477 Myc-DDK-tagged ORF clone of viral ORF for HA2 [Influenza A virus (A/Puerto Rico/8/1934(H1N1))], codon optimized for human cell expression, YP_163736 10 ug
$450.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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