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NA (NC_002018) Virus Tagged ORF Clone

  • TrueORF®
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SKU
VC102468
Myc-DDK-tagged ORF clone of viral ORF for neuraminidase [Influenza A virus (A/Puerto Rico/8/1934(H1N1))], codon optimized for human cell expression, NP_040981
  $686.00
In Stock*
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Specifications
Specifications
Product Data
Type Virus Tagged ORF Clone
Target Symbol NA
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>The Viral ORF clone VC102468 represents NCBI reference of NP_040981 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s)

GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAACCCTAACCAAAAGATTATCACTATAGGATCAATTTGCCTGGTGGTTGGCTTGATCAGCCTGATCC
TCCAGATAGGGAATATTATCAGTATTTGGATTTCACACAGCATCCAGACCGGCTCCCAGAACCACACAGG
TATTTGCAACCAGAACATCATCACCTACAAGAATAGCACTTGGGTCAAGGATACGACAAGCGTCATTCTG
ACCGGCAACTCATCCCTCTGTCCTATTCGCGGGTGGGCCATATATTCCAAAGACAACAGTATTAGGATCG
GCAGCAAAGGAGATGTCTTCGTTATTCGCGAACCATTTATCTCATGTAGTCATCTGGAGTGCAGAACATT
TTTTTTGACCCAGGGTGCACTCCTCAACGATAGACACAGTAACGGTACCGTTAAAGATAGAAGTCCTTAC
CGAGCCCTGATGTCTTGCCCCGTCGGGGAAGCCCCAAGCCCCTATAACTCTCGGTTTGAAAGTGTTGCCT
GGTCAGCCTCAGCCTGTCACGACGGCATGGGCTGGCTGACCATCGGAATCTCTGGCCCGGACAATGGTGC
TGTGGCTGTGCTCAAGTATAATGGAATAATCACTGAAACTATAAAATCCTGGAGGAAGAAAATACTGCGG
ACTCAGGAGTCTGAATGCGCATGCGTGAATGGAAGCTGCTTCACAATTATGACAGATGGCCCAAGCGATG
GGCTTGCCAGCTATAAGATCTTCAAGATCGAAAAGGGCAAGGTTACTAAGTCAATTGAGCTGAACGCACC
TAATTCCCACTATGAAGAATGCTCATGCTACCCCGACACCGGCAAAGTCATGTGTGTTTGTCGAGATAAC
TGGCACGGGTCAAATCGGCCATGGGTCTCCTTCGACCAGAACCTCGACTATCAGATCGGTTACATCTGTA
GTGGCGTATTCGGAGACAACCCTAGACCAAAGGATGGCACAGGGTCTTGCGGCCCGGTGTACGTTGACGG
CGCTAACGGTGTCAAAGGCTTCAGTTATAGGTATGGCAACGGGGTGTGGATCGGACGAACCAAGAGCCAC
TCAAGCCGGCATGGATTTGAAATGATATGGGATCCCAACGGGTGGACCGAGACAGACAGTAAATTCTCCG
TTAGACAGGATGTTGTGGCCATGACCGACTGGTCCGGATATAGCGGAAGCTTCGTCCAGCACCCAGAACT
GACAGGGCTGGATTGCATCAGGCCCTGCTTCTGGGTGGAGCTGATCCGCGGAAGGCCTAAGGAGAAAACC
ATCTGGACTTCCGCCTCTTCCATCTCATTCTGTGGGGTTAACTCCGACACTGTGGACTGGTCCTGGCCTG
ATGGAGCGGAGCTGCCATTCACCATAGACAAG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>VC102468 representing NP_040981
Red=Cloning sites Green=Tags

MNPNQKIITIGSICLVVGLISLILQIGNIISIWISHSIQTGSQNHTGICNQNIITYKNSTWVKDTTSVIL
TGNSSLCPIRGWAIYSKDNSIRIGSKGDVFVIREPFISCSHLECRTFFLTQGALLNDRHSNGTVKDRSPY
RALMSCPVGEAPSPYNSRFESVAWSASACHDGMGWLTIGISGPDNGAVAVLKYNGIITETIKSWRKKILR
TQESECACVNGSCFTIMTDGPSDGLASYKIFKIEKGKVTKSIELNAPNSHYEECSCYPDTGKVMCVCRDN
WHGSNRPWVSFDQNLDYQIGYICSGVFGDNPRPKDGTGSCGPVYVDGANGVKGFSYRYGNGVWIGRTKSH
SSRHGFEMIWDPNGWTETDSKFSVRQDVVAMTDWSGYSGSFVQHPELTGLDCIRPCFWVELIRGRPKEKT
IWTSASSISFCGVNSDTVDWSWPDGAELPFTIDK

TRTRRLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene |
ACCN NC_002018
ORF Size 1362 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NC_002018.1, NP_040981
RefSeq ORF 1362 bp
Locus ID 956530
MW 50.1 kDa
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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