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E1 protein (NC_004102) Virus Tagged ORF Clone

  • TrueORF®
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SKU
VC102216
Myc-DDK-tagged ORF clone of viral ORF for E1 protein [Hepatitis C virus], codon optimized for human cell expression, NP_751920
$289.00 $300.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "E1 protein or NC_004102"

Specifications
Specifications
Product Data
Type Virus Tagged ORF Clone
Target Symbol E1 protein
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>The Viral ORF clone VC102216 represents NCBI reference of NP_751920 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s)

GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTACCAGGTCAGAAATAGCTCTGGGCTGTATCACGTGACAAATGATTGCCCGAACAGCTCTATTGTCT
ATGAGGCCGCTGACGCAATTCTCCACACCCCTGGCTGTGTGCCTTGTGTTCGGGAGGGCAATGCCTCTCG
CTGTTGGGTTGCCGTCACACCCACCGTCGCCACACGCGATGGAAAGCTGCCCACAACGCAGCTGAGGCGG
CACATTGACCTTCTGGTCGGTTCAGCTACGCTGTGCTCAGCCCTGTACGTCGGAGACCTGTGTGGCAGCG
TGTTTCTGGTCGGACAGCTCTTTACCTTCTCTCCAAGACGGCATTGGACCACCCAGGATTGTAACTGCAG
CATTTACCCGGGTCATATAACCGGCCATCGCATGGCTTGGGACATGATGATGAATTGGTCTCCTACGGCC
GCCCTGGTGGTTGCCCAATTGCTTAGGATCCCCCAGGCGATCATGGACATGATTGCTGGAGCCCATTGGG
GCGTTCTCGCGGGAATCGCCTACTTCAGTATGGTAGGGAACTGGGCAAAGGTGCTTGTGGTGTTGTTGTT
GTTTGCCGGCGTTGACGCT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>VC102216 representing NP_751920
Red=Cloning sites Green=Tags

MYQVRNSSGLYHVTNDCPNSSIVYEAADAILHTPGCVPCVREGNASRCWVAVTPTVATRDGKLPTTQLRR
HIDLLVGSATLCSALYVGDLCGSVFLVGQLFTFSPRRHWTTQDCNCSIYPGHITGHRMAWDMMMNWSPTA
ALVVAQLLRIPQAIMDMIAGAHWGVLAGIAYFSMVGNWAKVLVVLLLFAGVDA

TRTRRLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene |
ACCN NC_004102
ORF Size 579 bp
OTI Disclaimer The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NC_004102.1, NP_751920
RefSeq ORF 579 bp
MW 21.0 kDa
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"NC_004102" in other vectors (9)
SKU Description Size Price
VC102214 Myc-DDK-tagged ORF clone of viral ORF for protein F [Hepatitis C virus], codon optimized for human cell expression, NP_803170 10 ug
$289.00
VC102215 Myc-DDK-tagged ORF clone of viral ORF for core protein [Hepatitis C virus], codon optimized for human cell expression, NP_751919 10 ug
$450.00
VC102217 Myc-DDK-tagged ORF clone of viral ORF for E2 protein [Hepatitis C virus], codon optimized for human cell expression, NP_751921 10 ug
$289.00 $457.00
VC102218 Myc-DDK-tagged ORF clone of viral ORF for p7 protein [Hepatitis C virus], codon optimized for human cell expression, NP_751922 10 ug
$289.00
VC102219 Myc-DDK-tagged ORF clone of viral ORF for NS2 protein [Hepatitis C virus], codon optimized for human cell expression, NP_751923 10 ug
$289.00 $300.00
VC102220 Myc-DDK-tagged ORF clone of viral ORF for NS3 protease/helicase' [Hepatitis C virus], codon optimized for human cell expression, NP_803144 10 ug
$289.00 $588.00
VC102221 Myc-DDK-tagged ORF clone of viral ORF for NS4A protein [Hepatitis C virus], codon optimized for human cell expression, NP_751925 10 ug
$289.00
VC102222 Myc-DDK-tagged ORF clone of viral ORF for NS4B protein [Hepatitis C virus], codon optimized for human cell expression, NP_751926 10 ug
$289.00 $300.00
VC102223 Myc-DDK-tagged ORF clone of viral ORF for NS5A protein [Hepatitis C virus], codon optimized for human cell expression, NP_751927 10 ug
$289.00 $457.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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