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2A (NC_009996) Virus Tagged ORF Clone

  • TrueORF®
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SKU
VC102134
Myc-DDK-tagged ORF clone of viral ORF for 2A [Human rhinovirus C], codon optimized for human cell expression, YP_001552436
  $165.00
3 Weeks*
Bulk Requests & Clone Modifications

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Other products for "2A or NC_009996"

Specifications
Specifications
Product Data
Type Virus Tagged ORF Clone
Target Symbol 2A
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>The Viral ORF clone VC102134 represents NCBI reference of YP_001552436 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s)

GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGACCAAGCGACCTGTTCGTTCACACTGCCACCATGATCTACCGGAACGCCCACCTGACAAGTCTGA
ATGATAAAACTGTGCTGCTCGCTCTGAGCGCTGACCTCCAAGTTGACGCAGCTGATCAGCCCGGCCCTGA
CACTATCCCGCAGTGTGACTGTTTGGAGGGATGCTATTATTCAAAGTCTCTTGACAGATACATCCCTGTG
AAGCTGGAGGCCCACGATTGGTACCAGATAGAGGAGACTTGCTACTATCCCCAGCATATTCAATATAATA
TAATGATTGGGGAAGGCCACTGTCAACCTGGAGATTGTGGCGGAAAACTGCTCTGCACTCATGGGGTCAT
TGGAATTATTACCGCTGGCGGAGATAATCACGTCGCGTTCACCGACCTGCGCCCCTATAGCTGCCTCGAC
ACACATCAG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>VC102134 representing YP_001552436
Red=Cloning sites Green=Tags

MGPSDLFVHTATMIYRNAHLTSLNDKTVLLALSADLQVDAADQPGPDTIPQCDCLEGCYYSKSLDRYIPV
KLEAHDWYQIEETCYYPQHIQYNIMIGEGHCQPGDCGGKLLCTHGVIGIITAGGDNHVAFTDLRPYSCLD
THQ

TRTRRLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene |
ACCN NC_009996
ORF Size 429 bp
OTI Disclaimer The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NC_009996.1, YP_001552436
RefSeq ORF 429 bp
MW 15.8 kDa
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"NC_009996" in other vectors (9)
SKU Description Size Price
VC102130 Myc-DDK-tagged ORF clone of viral ORF for VP4 [Human rhinovirus C], codon optimized for human cell expression, YP_001552432. Note: ORF is codon optimized 10 ug
$165.00
VC102131 Myc-DDK-tagged ORF clone of viral ORF for VP2 [Human rhinovirus C], codon optimized for human cell expression, YP_001552433 10 ug
$330.00
VC102132 Myc-DDK-tagged ORF clone of viral ORF for VP3 [Human rhinovirus C], codon optimized for human cell expression, YP_001552434 10 ug
$330.00
VC102133 Myc-DDK-tagged ORF clone of viral ORF for VP1 [Human rhinovirus C], codon optimized for human cell expression, YP_001552435 10 ug
$330.00
VC102135 Myc-DDK-tagged ORF clone of viral ORF for 2B [Human rhinovirus C], codon optimized for human cell expression, YP_001552437 10 ug
$165.00
VC102136 Myc-DDK-tagged ORF clone of viral ORF for 2C [Human rhinovirus C], codon optimized for human cell expression, YP_001552438 10 ug
$289.00 $300.00
VC102137 Myc-DDK-tagged ORF clone of viral ORF for 3A [Human rhinovirus C], codon optimized for human cell expression, YP_001552439 10 ug
$289.00
VC102138 Myc-DDK-tagged ORF clone of viral ORF for 3B [Human rhinovirus C], codon optimized for human cell expression, YP_001552440 10 ug
$165.00
VC102139 Myc-DDK-tagged ORF clone of viral ORF for 3C [Human rhinovirus C], codon optimized for human cell expression, YP_001552441 10 ug
$330.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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