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VP3 (NC_015150) Virus Tagged ORF Clone

  • TrueORF®
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SKU
VC102091
Myc-DDK-tagged ORF clone of viral ORF for VP3 [Human polyomavirus 9], codon optimized for human cell expression, YP_004243704
  $330.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Virus Tagged ORF Clone
Target Symbol VP3
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>The Viral ORF clone VC102091 represents NCBI reference of YP_004243704 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s)

GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCCTGACGCCTTGGTTTCCTCAGGTGGATTATCTGTTCCCCGGACTGTCCAGCTTTAGTTACTACC
TGAATGCTGCACTGGATTGGGGCGAAAGTCTCTTCCACGCCGTCGGTCGAGAGATCTGGAGGAACATCAT
GCGGCAGGCAACACAACAGATTGGCTACACTTCAAGAGCACTTGCCGTTCGAGGAACAAATGAGTTTCAG
CATATGCTCGCGCAGATCGCGGAAAATGCCCGCTGGGCTCTTACTAACGGACCTATTCACATCTATAGTA
GCGTCGAGGAGTATTACAGGGGATTGCCTAGCGTGAATCCAATTCAGCTGAGACAGCAGTATAGGTCAAG
AGGCGAGCTTCCCCCCACCCGAGAGCAATTCGAGTACCAGGAACAGGTGCGCCTGCGGAGAGAAATTGGC
GGGTCAGAGCCCCGGAGTGGGCACTATGTTCAGCATTACGCAGCCCCTGGCGGGGCTAATCAGAGGGTGA
GTCAGGATTGGATGCTGCCCCTTATTCTCGGTCTGTACGGGGACATCACACCCACATGGGAGAAAGAGCT
GTCCAAGTTGGAGAAAGAAGAGTACGGACCCCCCAAAAAAAAAGCTCGGGTGCGGTCAATGAGCTGTAAA
AAGAACCTGTCCAATACACGCAGCAGGAGCCAGACACCCTGTCAACGCAGGGGGAGATCCTCTCGGTCA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>VC102091 representing YP_004243704
Red=Cloning sites Green=Tags

MALTPWFPQVDYLFPGLSSFSYYLNAALDWGESLFHAVGREIWRNIMRQATQQIGYTSRALAVRGTNEFQ
HMLAQIAENARWALTNGPIHIYSSVEEYYRGLPSVNPIQLRQQYRSRGELPPTREQFEYQEQVRLRREIG
GSEPRSGHYVQHYAAPGGANQRVSQDWMLPLILGLYGDITPTWEKELSKLEKEEYGPPKKKARVRSMSCK
KNLSNTRSRSQTPCQRRGRSSRS

TRTRRLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene |
ACCN NC_015150
ORF Size 699 bp
OTI Disclaimer The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NC_015150.1, YP_004243704
RefSeq ORF 699 bp
Locus ID 10352033
MW 26.9 kDa
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"NC_015150" in other vectors (4)
SKU Description Size Price
VC102089 Myc-DDK-tagged ORF clone of viral ORF for VP1 [Human polyomavirus 9], codon optimized for human cell expression, YP_004243705 10 ug
$503.00
VC102090 Myc-DDK-tagged ORF clone of viral ORF for VP2 [Human polyomavirus 9], codon optimized for human cell expression, YP_004243703 10 ug
$503.00
VC102092 Myc-DDK-tagged ORF clone of viral ORF for large T antigen [Human polyomavirus 9], codon optimized for human cell expression, YP_004243706 10 ug
$996.00
VC102093 Myc-DDK-tagged ORF clone of viral ORF for small T antigen [Human polyomavirus 9], codon optimized for human cell expression, YP_004243707 10 ug
$330.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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