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7.5 kDa protein (NC_000883) Virus Tagged ORF Clone

  • TrueORF®
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SKU
VC102084
Myc-DDK-tagged ORF clone of viral ORF for 75 kDa protein [Human parvovirus B19], codon optimized for human cell expression, YP_004928145
  $165.00
3 Weeks*
Bulk Requests & Clone Modifications

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Other products for "7.5 kDa protein or NC_000883"

Specifications
Specifications
Product Data
Type Virus Tagged ORF Clone
Target Symbol 7.5 kDa protein
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>The Viral ORF clone VC102084 represents NCBI reference of YP_004928145 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s)

GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCAGATGCCATCTACACAGACCAGTAAGCCCCCACAGCTTTCTCAGACCCCAGTTTCTGCAGCCGTAG
TAGTGAAAGCCCTCAAGAATTCAGTGAAAGCCGCTTTCCTCACATCCAGCCCACAGGCGCCTGGGACACT
CAAGCCAAGAGCCCTGGTGAGGCCTTCTCCCGGACCAGTTCAGGAAAACCATCTCTCTGAAGCGCAGTTT
CCGCCAAAGCTG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>VC102084 representing YP_004928145
Red=Cloning sites Green=Tags

MQMPSTQTSKPPQLSQTPVSAAVVVKALKNSVKAAFLTSSPQAPGTLKPRALVRPSPGPVQENHLSEAQF
PPKL

TRTRRLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene |
ACCN NC_000883
ORF Size 222 bp
OTI Disclaimer The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NC_000883.2, YP_004928145
RefSeq ORF 222 bp
Locus ID 11293634
MW 7.8 kDa
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"NC_000883" in other vectors (5)
SKU Description Size Price
VC102080 Myc-DDK-tagged ORF clone of viral ORF for non-structural protein NS1 [Human parvovirus B19], codon optimized for human cell expression, YP_004928144 10 ug
$983.00
VC102081 Myc-DDK-tagged ORF clone of viral ORF for capsid protein 1 [Human parvovirus B19], codon optimized for human cell expression, YP_004928146 10 ug
$787.00
VC102082 Myc-DDK-tagged ORF clone of viral ORF for capsid protein 2 [Human parvovirus B19], codon optimized for human cell expression, YP_004928148 10 ug
$567.00
VC102083 Myc-DDK-tagged ORF clone of viral ORF for 11 kDa protein [Human parvovirus B19], codon optimized for human cell expression, YP_004928149 10 ug
$165.00
VC102085 Myc-DDK-tagged ORF clone of viral ORF for protein X [Human parvovirus B19], codon optimized for human cell expression, YP_004928147 10 ug
$165.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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