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E5 (NC_001531) Virus Tagged ORF Clone

  • TrueORF®
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SKU
VC101961
Myc-DDK-tagged ORF clone of viral ORF for E5, partial [Human papillomavirus type 5], codon optimized for human cell expression, NP_041370
  $330.00
3 Weeks*
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Specifications
Specifications
Product Data
Type Virus Tagged ORF Clone
Target Symbol E5
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>The Viral ORF clone VC101961 represents NCBI reference of NP_041370 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s)

GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

CTCCATGTTCAGACTGCCCACCACATTAAAACCGCCACCACGAACCGAAACCAGCGCAAAAAAGTGAGAA
CAGAGGCCCTGCAGCAAGTCAAAGAGATCGCCAATGCCGCAAAAGCGATCAAGGTCCCAACTCCTGTTCC
CGTGTCAGTACCCGTCGCCGTGCAAGTGCCCAATCCCCACCACAGCGTACACCACCAGGTCCCTGTGCAC
GTCGCGCACCAGGATAGTGGCCCGTATAAGCAGATTGCAATCCAGCGCAAGGTGCCTAACCACCTGCAGA
AAGGAAGATGGAAGGTCACTCAAGCAGCCATCAAGGTCACACTTCATCTGCTGCTCCTTCATCATACAAC
CGTTACCGCCGGCACTAGTCGCAAGTTCAATAATCAGCGGGGGCCAAGAGTCGAACGGGTGACCCGGAGA
GAACCATGGGGCGAGCGCGCGGCTACTAGGAAGGTGATTCTGCTGCTGCTGCCACGCCCACAGACAGTCA
CACGCGGAGTGTGC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>VC101961 representing NP_041370
Red=Cloning sites Green=Tags

LHVQTAHHIKTATTNRNQRKKVRTEALQQVKEIANAAKAIKVPTPVPVSVPVAVQVPNPHHSVHHQVPVH
VAHQDSGPYKQIAIQRKVPNHLQKGRWKVTQAAIKVTLHLLLLHHTTVTAGTSRKFNNQRGPRVERVTRR
EPWGERAATRKVILLLLPRPQTVTRGVC

TRTRRLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene |
ACCN NC_001531
ORF Size 504 bp
OTI Disclaimer The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NC_001531.1, NP_041370
RefSeq ORF 504 bp
Locus ID 1489052
MW 18.9 kDa
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"NC_001531" in other vectors (6)
SKU Description Size Price
VC101956 Myc-DDK-tagged ORF clone of viral ORF for transforming protein [Human papillomavirus type 5], codon optimized for human cell expression, NP_041365 10 ug
$225.00
VC101957 Myc-DDK-tagged ORF clone of viral ORF for transforming protein [Human papillomavirus type 5], codon optimized for human cell expression, NP_041366 10 ug
$225.00
VC101958 Myc-DDK-tagged ORF clone of viral ORF for replication protein [Human papillomavirus type 5], codon optimized for human cell expression, NP_041367 10 ug
$902.00
VC101959 Myc-DDK-tagged ORF clone of viral ORF for regulatory protein [Human papillomavirus type 5], codon optimized for human cell expression, NP_041368 10 ug
$718.00
VC101962 Myc-DDK-tagged ORF clone of viral ORF for minor capsid protein [Human papillomavirus type 5], codon optimized for human cell expression, NP_041371 10 ug
$530.00
VC101963 Myc-DDK-tagged ORF clone of viral ORF for major capsid protein [Human papillomavirus type 5], codon optimized for human cell expression, NP_041372 10 ug
$528.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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