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2C (NC_001472) Virus Tagged ORF Clone

  • TrueORF®
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SKU
VC100734
Myc-DDK-tagged ORF clone of viral ORF for 2C [Human enterovirus B], codon optimized for human cell expression, NP_740543
  $330.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Virus Tagged ORF Clone
Target Symbol 2C
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>The Viral ORF clone VC100734 represents NCBI reference of NP_740543 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s)

GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAACTCCGGATGGCTGAAAAAGTTTACGGAGATGACAAATGCATGTAAGGGGATGGAATGGATTGCAA
TAAAAATTCAGAAATTTATCGAATGGCTCAAAGTGAAGATCCTCCCAGAGGTGAAAGAAAAGCACGAATT
CCTCAACAGACTGAAGCAGCTGCCACTGCTGGAAAGCCAGATCGCAACTATAGAACAGAGCGCACCTTCC
CAGTCTGACCAGGAGCAGCTTTTTTCTAATGTGCAGTATTTCGCACACTACTGCCGGAAGTACGCCCCAC
TGTACGCCGCCGAGGCCAAGAGGGTCTTTTCTTTGGAAAAAAAGATGAGCAACTATATCCAGTTCAAATC
AAAGTGCAGGATCGAGCCCGTCTGTCTGCTTCTCCACGGCTCCCCTGGGGCTGGCAAGAGCGTCGCAACG
AATCTGATCGGCAGGAGTCTGGCCGAGAAACTCAATTCTAGCGTTTATTCACTGCCTCCTGATCCTGATC
ATTTTGACGGGTATAAGCAACAGGCTGTGGTTATTATGGACGACCTCTGTCAAAATCCTGACGGTAAGGA
TGTCAGCCTCTTCTGTCAGATGGTATCCTCTGTCGACTTCGTCCCCCCCATGGCCGCTCTCGAAGAAAAG
GGGATCCTGTTCACCAGCCCTTTCGTCCTGGCGTCTACCAACGCTGGTTCAATCAACGCTCCAACGGTCT
CCGACTCTCGGGCGCTGGCCCGGAGGTTTCACTTTGATATGAACATCGAGGTGATATCTATGTACTCACA
GAATGGGAAGATAAACATGCCAATGTCCGTTAAGACTTGTGACGAAGAGTGCTGTCCTGTGAATTTTAAG
AAGTGTTGTCCATTGGTCTGCGGGAAAGCCATCCAGTTCATCGACCGAAGAACTCAAGTACGATATTCCC
TGGATATGCTTGTGACCGAGATGTTCAGAGAGTACAATCATCGGCACTCTGTCGGAGCTACTCTCGAAGC
ACTTTTTCAG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>VC100734 representing NP_740543
Red=Cloning sites Green=Tags

MNSGWLKKFTEMTNACKGMEWIAIKIQKFIEWLKVKILPEVKEKHEFLNRLKQLPLLESQIATIEQSAPS
QSDQEQLFSNVQYFAHYCRKYAPLYAAEAKRVFSLEKKMSNYIQFKSKCRIEPVCLLLHGSPGAGKSVAT
NLIGRSLAEKLNSSVYSLPPDPDHFDGYKQQAVVIMDDLCQNPDGKDVSLFCQMVSSVDFVPPMAALEEK
GILFTSPFVLASTNAGSINAPTVSDSRALARRFHFDMNIEVISMYSQNGKINMPMSVKTCDEECCPVNFK
KCCPLVCGKAIQFIDRRTQVRYSLDMLVTEMFREYNHRHSVGATLEALFQ

TRTRRLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene |
ACCN NC_001472
ORF Size 990 bp
OTI Disclaimer The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NC_001472.1, NP_740543
RefSeq ORF 990 bp
MW 37.4 kDa
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"NC_001472" in other vectors (9)
SKU Description Size Price
VC100728 Myc-DDK-tagged ORF clone of viral ORF for 1A(VP4) [Human enterovirus B], codon optimized for human cell expression, NP_740537 10 ug
$165.00
VC100729 Myc-DDK-tagged ORF clone of viral ORF for 1B(VP2) [Human enterovirus B], codon optimized for human cell expression, NP_740538 10 ug
$330.00
VC100730 Myc-DDK-tagged ORF clone of viral ORF for 1C(VP3) [Human enterovirus B], codon optimized for human cell expression, NP_740539 10 ug
$330.00
VC100731 Myc-DDK-tagged ORF clone of viral ORF for 1D(VP1) [Human enterovirus B], codon optimized for human cell expression, NP_740540 10 ug
$330.00
VC100732 Myc-DDK-tagged ORF clone of viral ORF for 2A [Human enterovirus B], codon optimized for human cell expression, NP_740541 10 ug
$165.00
VC100733 Myc-DDK-tagged ORF clone of viral ORF for 2B [Human enterovirus B], codon optimized for human cell expression, NP_740542 10 ug
$165.00
VC100735 Myc-DDK-tagged ORF clone of viral ORF for 3A [Human enterovirus B], codon optimized for human cell expression, NP_740544 10 ug
$165.00
VC100736 Myc-DDK-tagged ORF clone of viral ORF for 3B(VPg) [Human enterovirus B], codon optimized for human cell expression, NP_740545 10 ug
$165.00
VC100737 Myc-DDK-tagged ORF clone of viral ORF for 3C [Human enterovirus B], codon optimized for human cell expression, NP_740546 10 ug
$289.00 $300.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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