2B (NC_013114) Virus Tagged ORF Clone

  • TrueORF®
SKU
VC100709
Myc-DDK-tagged ORF clone of viral ORF for 2B [Human enterovirus 98], codon optimized for human cell expression, YP_003104780
  $165.00
3 Weeks*
Specifications
Specifications
Product Data
Type Virus Tagged ORF Clone
Target Symbol 2B
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>The Viral ORF clone VC100709 represents NCBI reference of YP_003104780 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s)

GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGGGTCCGGGACTACGTTGAGCAACTGGGGAACGCATTCGGAAGTGGCTTCACCAATCAGATCTGTG
AACAGGTGAATCTTCTCAAGGAAACCCTGGTCGGCCAGGACAGTATTCTGGAAAAGTCACTCAAAGCTCT
GGTGAAGATTATCTCAGCATTGGTCATCGTGGTGCGGAACCATGACGATCTGGTAACCGTGACCGCGACA
CTGGCCCTGATCGGTTGTTCTGCTAGCCCCTGGAGATGGCTGAAGCAGAAAGTCTCTCAGTATTACGGGA
TCCCCATGGCAGAGAGACAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>VC100709 representing YP_003104780
Red=Cloning sites Green=Tags

MGVRDYVEQLGNAFGSGFTNQICEQVNLLKETLVGQDSILEKSLKALVKIISALVIVVRNHDDLVTVTAT
LALIGCSASPWRWLKQKVSQYYGIPMAERQ

TRTRRLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene |
ACCN NC_013114
ORF Size 300 bp
OTI Disclaimer The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NC_013114.1, YP_003104780
RefSeq ORF 300 bp
MW 11.1 kDa
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Documents
Resources
"NC_013114" in other vectors (9)
SKU Description Size Price
VC100704 Myc-DDK-tagged ORF clone of viral ORF for VP4 [Human enterovirus 98], codon optimized for human cell expression, YP_003104775 10 ug
$165.00
VC100705 Myc-DDK-tagged ORF clone of viral ORF for VP2 [Human enterovirus 98], codon optimized for human cell expression, YP_003104776 10 ug
$330.00
VC100706 Myc-DDK-tagged ORF clone of viral ORF for VP3 [Human enterovirus 98], codon optimized for human cell expression, YP_003104777 10 ug
$330.00
VC100707 Myc-DDK-tagged ORF clone of viral ORF for VP1 [Human enterovirus 98], codon optimized for human cell expression, YP_003104778 10 ug
$330.00
VC100708 Myc-DDK-tagged ORF clone of viral ORF for 2A [Human enterovirus 98], codon optimized for human cell expression, YP_003104779 10 ug
$165.00
VC100710 Myc-DDK-tagged ORF clone of viral ORF for 2C [Human enterovirus 98], codon optimized for human cell expression, YP_003104781 10 ug
$330.00
VC100711 Myc-DDK-tagged ORF clone of viral ORF for 3A [Human enterovirus 98], codon optimized for human cell expression, YP_003104782 10 ug
$165.00
VC100712 Myc-DDK-tagged ORF clone of viral ORF for 3B [Human enterovirus 98], codon optimized for human cell expression, YP_003104783 10 ug
$165.00
VC100713 Myc-DDK-tagged ORF clone of viral ORF for 3C [Human enterovirus 98], codon optimized for human cell expression, YP_003104784 10 ug
$289.00 $330.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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