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HCoV-NL63 ORF3 Gene Tagged ORF Clone

  • TrueORF®
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SKU
VC100594
Myc-DDK-tagged ORF clone for protein 3 [Human coronavirus NL63], codon optimized for human cell expression, YP_003768
  $330.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Virus Tagged ORF Clone
Target Symbol protein 3
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>The Viral ORF clone VC100594 represents NCBI reference of YP_003768 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s)

GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCCTTTGGAGGTCTGTTTCAGCTCACCCTGGAAAGTACTATTAACAAAAGCGTGGCCAACCTCAAGC
TCCCACCTCACGACGTGACAGTGCTGAGAGATAACCTGAAGCCTGTCACTACTCTGTCTACCATCACCGC
CTACCTGTTGGTGTCCCTTTTCGTTACCTACTTTGCATTGTTCAAGCCTCTGACCGCAAGAGGCAGGGTT
GCATGTTTCGTCCTGAAACTCCTCACATTGTTTGTGTATGTTCCACTGCTGGTGCTGTTCGGGATGTATC
TGGACAGTTTCATCATTTTCTCCACCCTCCTGTTCCGCTTTATCCACGTGGGCTATTATGCCTACCTGTA
CAAAAACTTTTCCTTCGTCTTGTTTAACGTGACGAAACTCTGTTTCGTGTCAGGCAAGTGTTGGTATCTT
GAGCAGTCCTTCTACGAAAATAGGTTCGCTGCCATATATGGGGGGGATCACTACGTAGTGCTGGGCGGCG
AGACCATCACCTTTGTCAGCTTTGACGACCTGTACGTGGCCATTCGCGGCTCATGCGAGAAGAATCTTCA
ACTGATGCGAAAAGTGGACCTGTATAACGGAGCCGTAATTTACATTTTCGCCGAAGAACCCGTCGTGGGA
ATTGTCTACTCTTCACAACTGTACGAGGATGTACCCAGTATTAAC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>VC100594 representing YP_003768
Red=Cloning sites Green=Tags

MPFGGLFQLTLESTINKSVANLKLPPHDVTVLRDNLKPVTTLSTITAYLLVSLFVTYFALFKPLTARGRV
ACFVLKLLTLFVYVPLLVLFGMYLDSFIIFSTLLFRFIHVGYYAYLYKNFSFVLFNVTKLCFVSGKCWYL
EQSFYENRFAAIYGGDHYVVLGGETITFVSFDDLYVAIRGSCEKNLQLMRKVDLYNGAVIYIFAEEPVVG
IVYSSQLYEDVPSIN

TRTRRLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene |
ACCN NC_005831
ORF Size 675 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NC_005831.2, YP_003768
RefSeq ORF 675 bp
Locus ID 2943500
MW 25.7 kDa
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"NC_005831" in other vectors (4)
SKU Description Size Price
VC100593 Myc-DDK-tagged ORF clone for spike protein [Human coronavirus NL63], codon optimized for human cell expression, YP_003767 10 ug
$589.00 $1,160.00
VC100595 Myc-DDK-tagged ORF clone for envelope protein [Human coronavirus NL63], codon optimized for human cell expression, YP_003769 10 ug
$289.00
VC100596 Myc-DDK-tagged ORF clone for membrane protein [Human coronavirus NL63], codon optimized for human cell expression, YP_003770 10 ug
$289.00 $300.00
VC100597 Myc-DDK-tagged ORF clone for nucleocapsid protein [Human coronavirus NL63], codon optimized for human cell expression, YP_003771 10 ug
$686.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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