OriGene Logo

Customer Login

Checkout as a new customer

Creating an account has many benefits:

  • See order and shipping status
  • Track order history
  • Check out faster
Create an Account

HCoV-HKU1 nsp14 Gene Tagged ORF Clone

  • TrueORF®
Be the first to review this product
SKU
VC100586
Myc-DDK-tagged ORF clone for nsp14 [Human coronavirus HKU1], codon optimized for human cell expression, YP_460021
  $535.00
3 Weeks*
Bulk Requests & Clone Modifications

Frequently Bought Together (3)

TA592569 DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
100 ul
$519.00
CC100003 DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
5 x 200 ul
$170.00
PS100001 pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
10 ug
$670.00

Other products for "nsp14 or NC_006577"

Specifications
Specifications
Product Data
Type Virus Tagged ORF Clone
Target Symbol nsp14
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>The Viral ORF clone VC100586 represents NCBI reference of YP_460021 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s)

GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTGTACCACTAACCTCTTCAAGGACTGCTCCAAGAGCTGCCTGGGATATCATCCTGCACATGCACCTT
CTTTTCTGGCTGTCGACGATAAGTATAAAGTTAACGAAAACCTGGCCGTGAACCTGAACATTTGTGAACC
CGTCCTGACTTATTCAAGGTTGATTAGCTTGATGGGCTTCAAGCTGGACCTCACACTCGACGGCTACTCT
AAACTCTTCATTACCAAGGACGAGGCCATCAAGCGGGTGAGGGGTTGGGTGGGCTTTGACGTCGAGGGGG
CGCACGCGACACGAGAAAACATCGGAACTAACTTTCCACTCCAGATAGGTTTCTCTACCGGAGTGGATTT
CGTGGTGGAGGCAACCGGTCTGTTTGCAGAAAGGGATTGTTACACGTTCAAAAAGACAGTCGCCAAGGCT
CCCCCCGGTGAGAAGTTCAAGCACCTTATTCCACTTATGTCCAAGGGACAAAAGTGGGACATCGTGCGCA
TCAGAATCGTTCAGATGCTGTCAGATTACCTGCTGGATTTGTCAGACTCCGTGGTCTTTATCACCTGGTC
AGCCTCCTTCGAACTTACTTGCTTGCGGTACTTCGCCAAGCTCGGGCGAGAGCTGAACTGCAACGTATGC
TCAAACCGCGCCACATGTTACAACAGCAGAACAGGCTACTATGGATGCTGGCGCCATAGCTATACCTGTG
ATTACGTATATAACCCGCTGATAGTTGATATCCAGCAGTGGGGTTATACCGGAAGTCTGACCTCAAACCA
CGATATCATCTGTAATGTCCACAAGGGCGCACACGTAGCGTCCGCTGATGCCATTATGACGAGGTGTTTG
GCAATCTATGATTGCTTCTGCAAGAGCGTAAATTGGAACTTGGAATACCCAATTATCAGTAACGAGGTGA
GCATCAATACCTCATGCCGCCTGCTGCAAAGAGTGATGCTGAAAGCTGCTATGCTCTGTAATAGGTATAA
TTTGTGTTATGATATCGGAAACCCGAAAGGACTGGCCTGTGTTAAGGATTACGAGTTTAAGTTCTACGAT
GCCTTCCCCGTCGCTAAATCCGTGAAGCAGCTTTTTTATGTCTACGACGTGCACAAAGATAATTTCAAGG
ATGGGCTCTGCATGTTCTGGAACTGCAACGTCGATAAGTATCCTAGCAACAGCATCGTGTGTCGGTTCGA
CACCCGGGTCCTGAATAAATTGAATCTGCCCGGATGTAACGGCGGTAGCCTGTATGTAAATAAACACGCA
TTTCACACAAACCCATTTACCAGAACCGTCTTTGAGAATCTGAAGCCCATGCCATTTTTCTACTACTCCG
ATACACCATGTGTTTACGTGGACGGGTTGGAGTCCAAGCAGGTGGATTATGTGCCACTGCGGTCAGCCAC
TTGTATTACCCGATGTAACCTCGGAGGAGCTGTGTGTAGTAAGCACGCTGAGGAATACTGTAACTACCTG
GAGAGCTATAACATCGTGACTACAGCCGGCTTTACCTTCTGGGTGTACAAGAACTTCGATTTTTACAATT
TGTGGAACACGTTTACAACACTCCAG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>VC100586 representing YP_460021
Red=Cloning sites Green=Tags

MCTTNLFKDCSKSCLGYHPAHAPSFLAVDDKYKVNENLAVNLNICEPVLTYSRLISLMGFKLDLTLDGYS
KLFITKDEAIKRVRGWVGFDVEGAHATRENIGTNFPLQIGFSTGVDFVVEATGLFAERDCYTFKKTVAKA
PPGEKFKHLIPLMSKGQKWDIVRIRIVQMLSDYLLDLSDSVVFITWSASFELTCLRYFAKLGRELNCNVC
SNRATCYNSRTGYYGCWRHSYTCDYVYNPLIVDIQQWGYTGSLTSNHDIICNVHKGAHVASADAIMTRCL
AIYDCFCKSVNWNLEYPIISNEVSINTSCRLLQRVMLKAAMLCNRYNLCYDIGNPKGLACVKDYEFKFYD
AFPVAKSVKQLFYVYDVHKDNFKDGLCMFWNCNVDKYPSNSIVCRFDTRVLNKLNLPGCNGGSLYVNKHA
FHTNPFTRTVFENLKPMPFFYYSDTPCVYVDGLESKQVDYVPLRSATCITRCNLGGAVCSKHAEEYCNYL
ESYNIVTTAGFTFWVYKNFDFYNLWNTFTTLQ

TRTRRLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene |
ACCN NC_006577
ORF Size 1566 bp
OTI Disclaimer The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NC_006577.2, YP_460021
RefSeq ORF 1566 bp
MW 59.7 kDa
Reviews
Write Review
 
 
 
 
 

Be the first to review this product

Please note:
Only reviews with images are eligible for a $20/20€ Amazon gift card.
Reviews without images are eligible for a $10/10€ Amazon gift card.
For more details about the terms and conditions, please visit the promotion page.

Review image
Documents
Resources
"NC_006577" in other vectors (18)
SKU Description Size Price
VC100570 Myc-DDK-tagged ORF clone for hemagglutinin-esterase glycoprotein [Human coronavirus HKU1], codon optimized for human cell expression, YP_173237 10 ug
$289.00 $457.00
VC100572 Myc-DDK-tagged ORF clone for non-structural protein [Human coronavirus HKU1], codon optimized for human cell expression, YP_173239 10 ug
$165.00
VC100573 Myc-DDK-tagged ORF clone for small membrane protein [Human coronavirus HKU1], codon optimized for human cell expression, YP_173240 10 ug
$165.00
VC100574 Myc-DDK-tagged ORF clone for membrane glycoprotein [Human coronavirus HKU1], codon optimized for human cell expression, YP_173241 10 ug
$289.00 $300.00
VC100575 Myc-DDK-tagged ORF clone for nucleocapsid phosphoprotein [Human coronavirus HKU1], codon optimized for human cell expression, YP_173242 10 ug
$686.00
VC100576 Myc-DDK-tagged ORF clone for nucleocapsid phosphoprotein 2 [Human coronavirus HKU1], codon optimized for human cell expression, YP_173243 10 ug
$330.00
VC100577 Myc-DDK-tagged ORF clone for nsp1 [Human coronavirus HKU1], codon optimized for human cell expression, YP_460018 10 ug
$330.00
VC100578 Myc-DDK-tagged ORF clone for nsp2 [Human coronavirus HKU1], codon optimized for human cell expression, YP_459934 10 ug
$602.00
VC100579 Myc-DDK-tagged ORF clone for nsp6 (hydrophobic domain) [Human coronavirus HKU1], codon optimized for human cell expression, YP_460019 10 ug
$330.00
VC100580 Myc-DDK-tagged ORF clone for nsp5 [Human coronavirus HKU1], codon optimized for human cell expression, YP_459936 10 ug
$330.00
VC100581 Myc-DDK-tagged ORF clone for nsp7 [Human coronavirus HKU1], codon optimized for human cell expression, YP_459938 10 ug
$165.00
VC100582 Myc-DDK-tagged ORF clone for nsp8 [Human coronavirus HKU1], codon optimized for human cell expression, YP_460020 10 ug
$330.00
VC100583 Myc-DDK-tagged ORF clone for nsp9 [Human coronavirus HKU1], codon optimized for human cell expression, YP_459943 10 ug
$165.00
VC100584 Myc-DDK-tagged ORF clone for nsp10 [Human coronavirus HKU1], codon optimized for human cell expression, YP_459939 10 ug
$165.00
VC100585 Myc-DDK-tagged ORF clone for nsp13 [Human coronavirus HKU1], codon optimized for human cell expression, YP_459942 10 ug
$618.00
VC100587 Myc-DDK-tagged ORF clone for nsp15 [Human coronavirus HKU1], codon optimized for human cell expression, YP_460022 10 ug
$503.00
VC100588 Myc-DDK-tagged ORF clone for nsp16 [Human coronavirus HKU1], codon optimized for human cell expression, YP_460023 10 ug
$330.00
VC100591 Myc-DDK-tagged ORF clone for nsp4 [Human coronavirus HKU1], codon optimized for human cell expression, YP_459935 10 ug
$503.00

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Related Products