eIF2B epsilon (EIF2B5) (NM_003907) Human Untagged Clone
SKU
SC324640
EIF2B5 (untagged)-Human eukaryotic translation initiation factor 2B, subunit 5 epsilon, 82kDa (EIF2B5)
$991.00
In Stock*
Specifications
| Product Data | |
| Type | Human Untagged Clone |
|---|---|
| Target Symbol | eIF2B epsilon |
| Synonyms | CACH; CLE; EIF-2B; EIF2Bepsilon; LVWM |
| Vector | pCMV6-AC |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Restriction Sites | Please inquire | |
| ACCN | NM_003907 |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_003907.2, NP_003898.2 |
| RefSeq Size | 2898 bp |
| RefSeq ORF | 2166 bp |
| Locus ID | 8893 |
| UniProt ID | Q13144 |
| Cytogenetics | 3q27.1 |
| Summary | This gene encodes one of five subunits of eukaryotic translation initiation factor 2B (EIF2B), a GTP exchange factor for eukaryotic initiation factor 2 and an essential regulator for protein synthesis. Mutations in this gene and the genes encoding other EIF2B subunits have been associated with leukoencephalopathy with vanishing white matter. provided by RefSeq, Nov 2009 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_003907" in other vectors (5)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC202322 | EIF2B5 (Myc-DDK-tagged)-Human eukaryotic translation initiation factor 2B, subunit 5 epsilon, 82kDa (EIF2B5) | 10 ug |
$990.00
|
|
| RC202322L3 | Lenti ORF clone of Human eukaryotic translation initiation factor 2B, subunit 5 epsilon, 82kDa (EIF2B5), Myc-DDK-tagged | 10 ug |
$1,290.00
|
|
| RC202322L4 | Lenti ORF clone of Human eukaryotic translation initiation factor 2B, subunit 5 epsilon, 82kDa (EIF2B5), mGFP tagged | 10 ug |
$1,290.00
|
|
| RG202322 | EIF2B5 (tGFP-tagged) - Human eukaryotic translation initiation factor 2B, subunit 5 epsilon, 82kDa (EIF2B5) | 10 ug |
$789.00
$1,190.00
|
|
| SC107570 | EIF2B5 (untagged)-Human eukaryotic translation initiation factor 2B, subunit 5 epsilon, 82kDa (EIF2B5) | 10 ug |
$1,326.00
|
|
Citations
Transcriptome analysis of hypoxic cancer cells uncovers intron retention in EIF2B5 as a mechanism to inhibit translation
Brady, Wang, Radens et al
PLoS Biol. (2017) 15 (9), e2002623 DOI: 10.1371/journal.pbio.2002623
Brady, Wang, Radens et al
PLoS Biol. (2017) 15 (9), e2002623 DOI: 10.1371/journal.pbio.2002623
AI Summary: OriGene products contributed to this study by providing expression plasmids used to investigate the functional impact of intron retention in EIF2B5 on translation and cell survival under hypoxia.
Snippet: Author summary Tumor hypoxia is a negative prognostic factor for many solid cancers. Cellular adaptation to hypoxia is largely mediated by widespread changes in gene expression and enables cancer cells to adjust and survive. Recently, alternative splicing has ... Cached;Cells were placed in incubator for 24 h, at which point the media was replaced. After an additional 24 h, cells were either ...
Showing 1-1 of 1 papers.
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