eIF2B epsilon (EIF2B5) (NM_003907) Human Tagged ORF Clone
- TrueORF®
SKU
RG202322
EIF2B5 (tGFP-tagged) - Human eukaryotic translation initiation factor 2B, subunit 5 epsilon, 82kDa (EIF2B5)
$789.00
$1,190.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | eIF2B epsilon |
| Synonyms | CACH; CLE; EIF-2B; EIF2Bepsilon; LVWM |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_003907 |
| ORF Size | 2163 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_003907.1, NP_003898.1 |
| RefSeq Size | 2898 bp |
| RefSeq ORF | 2166 bp |
| Locus ID | 8893 |
| UniProt ID | Q13144 |
| Cytogenetics | 3q27.1 |
| Summary | This gene encodes one of five subunits of eukaryotic translation initiation factor 2B (EIF2B), a GTP exchange factor for eukaryotic initiation factor 2 and an essential regulator for protein synthesis. Mutations in this gene and the genes encoding other EIF2B subunits have been associated with leukoencephalopathy with vanishing white matter. provided by RefSeq, Nov 2009 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_003907" in other vectors (5)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC202322 | EIF2B5 (Myc-DDK-tagged)-Human eukaryotic translation initiation factor 2B, subunit 5 epsilon, 82kDa (EIF2B5) | 10 ug |
$990.00
|
|
| RC202322L3 | Lenti ORF clone of Human eukaryotic translation initiation factor 2B, subunit 5 epsilon, 82kDa (EIF2B5), Myc-DDK-tagged | 10 ug |
$1,290.00
|
|
| RC202322L4 | Lenti ORF clone of Human eukaryotic translation initiation factor 2B, subunit 5 epsilon, 82kDa (EIF2B5), mGFP tagged | 10 ug |
$1,290.00
|
|
| SC107570 | EIF2B5 (untagged)-Human eukaryotic translation initiation factor 2B, subunit 5 epsilon, 82kDa (EIF2B5) | 10 ug |
$1,326.00
|
|
| SC324640 | EIF2B5 (untagged)-Human eukaryotic translation initiation factor 2B, subunit 5 epsilon, 82kDa (EIF2B5) | 10 ug |
$991.00
|
|
Citations
eIF2B localisation and its regulation during the integrated stress response is cell-type specific
Hanson, Ribeiro de Oliveira, Cross et al
iScience (2024)
Hanson, Ribeiro de Oliveira, Cross et al
iScience (2024)
Cited by 6
AI Summary: OriGene products, specifically the pCMV6-AC-tGFP plasmid vectors encoding EIF2B5 and EIF2S1, were used to generate and verify expression constructs for studying eIF2B localisation and regulation in this research.
Snippet: pCMV6-AC-tGFP plasmid vector encoding EIF2B5 and EIF2S1 was purchased from 697 OriGene (Rockville, Maryland, USA). The coding open reading frame of EIF2B5 from 698 the pCMV6-AC-tGFP vector was cloned into an empty pCMV6-AC-mGFP and empty 699 pCMV6-AC-mRFP vectors. The constructs were verified by sequencing
Cellular eIF2B subunit localization: implications for the integrated stress response and its control by small molecule drugs
Hodgson, Varanda, Ashe et al
Mol. Biol. Cell (2019) 30 (8), 942-958 DOI: 10.1091/mbc.E18-08-0538
Hodgson, Varanda, Ashe et al
Mol. Biol. Cell (2019) 30 (8), 942-958 DOI: 10.1091/mbc.E18-08-0538
AI Summary: OriGene’s pCMV6-AC-GFP and pCMV6-AC-RFP plasmid vectors encoding EIF2B5 and EIF2S1 were used to express and visualize eIF2B subunits in mammalian cells, enabling the study of their localization and role in the integrated stress response.
Snippet: pCMV6-AC-GFP plasmid vectors encoding EIF2B5 and EIF2S1 were purchased from OriGene (Rockville, MD). The coding open reading frame (ORF) of EIF2B5 from the pCMV6-AC-GFP vector was cloned into a pCMV6-AC-RFP vector (OriGene). … Site-directed mutagenesis was performed on the EIF2S1 plasmid to generate an S51A mutation using a QuikChange II Site-Directed Mutagenesis Kit (Agilent, Stockport, UK) in accordance with manufacturer’s instructions. …
Showing 1-4 of 4 papers.
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