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GRASP65 (GORASP1) (NM_031899) Human Untagged Clone

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SKU
SC323878
GORASP1 (untagged)-Human golgi reassembly stacking protein 1, 65kDa (GORASP1)
  $457.00
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol GRASP65
Synonyms GOLPH5; GRASP65; P65
Vector pCMV6-AC
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>OriGene sequence for NM_031899.2 CCCACGCGTCCGATCGAGCGCCGAGAGAGCGAGTCGGTGCTACTGGCGCCGGGTCGGTCC
GGGTAGGCGCAGCGGGACTGGACCTGGGTGCCGAGCGGAGCCGCTGCCATGGGCCTGGGC
GTCAGCGCTGAGCAGCCCGCAGGCGGCGCCGAGGGCTTCCACCTCCACGGGGTGCAGGAG
AACTCCCCAGCCCAGCAGGCGGGCCTGGAGCCCTACTTTGACTTCATCATCACCATTGGG
CACTCGAGGCTGAACAAGGAGAATGACACCCTGAAGGCACTACTGAAAGCCAATGTGGAG
AAGCCCGTGAAGCTGGAGGTGTTCAATATGAAGACCATGAGGGTGCGCGAGGTGGAGGTG
GTGCCCAGCAACATGTGGGGCGGCCAGGGCCTACTGGGTGCCAGTGTGCGCTTCTGCAGC
TTCCGCAGGGCCAGTGAGCAGGTGTGGCATGTGCTGGATGTGGAACCATCTTCACCTGCT
GCCCTTGCCGGCCTGCGCCCCTACACAGACTATGTGGTTGGTTCGGACCAGATTCTCCAG
GAGTCCGAGGACTTCTTTACGCTCATCGAGTCTCATGAGGGGAAGCCCTTGAAGCTGATG
GTGTATAACTCCAAGTCAGACTCCTGCCGGGAGGTGACTGTAACTCCCAACGCAGCCTGG
GGTGGAGAGGGCAGTCTGGGATGTGGCATTGGCTATGGGTATCTACACCGGATCCCAACT
CAGCCCCCCAGCTACCACAAGAAGCCACCTGGCACCCCACCACCTTCTGCTCTACCACTT
GGTGCCCCACCACCTGATGCTCTACCACCTGGACCCACCCCCGAGGACTCTCCTTCCCTG
GAGACAGGTTCCAGGCAGAGTGACTACATGGAGGCCCTGCTGCAGGCACCTGGCTCCTCC
ATGGAGGATCCCCTTCCTGGGCCTGGGAGTCCCAGCCACAGTGCTCCAGACCCTGATGGA
CTTCCCCATTTCATGGAGACTCCTCTTCAGCCCCCACCTCCAGTGCAGCGAGTTATGGAC
CCAGGCTTCCTGGACGTGTCGGGAATTTCTCTCTTGGACAACAGCAATGCCAGTGTGTGG
CCCAGCCTGCCCTCTTCCACAGAACTGACCACCACAGCTGTCTCAACCTCAGGGCCAGAG
GACATCTGCTCCAGCAGCAGTTCTCATGAGCGGGGTGGTGAGGCTACATGGTCTGGGTCA
GAGTTTGAGGTCTCCTTCCTGGACAGCCCAGGTGCCCAAGCCCAGGCGGACCACCTGCCT
CAGCTGACTCTTCCTGACAGTCTCACCTCTGCAGCCTCACCAGAAGATGGGCTGTCCGCC
GAGCTGCTTGAAGCTCAGGCTGAGGAGGAACCAGCAAGCACAGAGGGCCTAGATACTGGG
ACGGAGGCTGAGGGGCTGGACAGCCAGGCCCAGATCTCTACCACAGAATAACACCCTGGG
CTGTGACAAGGCCCATGATGACATTTCATGAGGCCCAGATGTGGGCAGGCAGCCCGGGCT
GCACTATGCGGTCAGGATTCCTTGCTGCTTTGGTGGGACATGAGGCCTCAGTGGGTGGTG
AGAGGGGGCCATGGGCCTTGTGGGAGGATATCTTTTGGCCGTGTACCCTCAGGTCCAGTG
CTTGGTCCTGCTCTGGTCACTGGGGCTTCCCAGGAGGCAGCAGGGTCTGGTCATAATCCA
GTGTGAAGATGAGGTGAGGAAGGAAGGGGTTGCTGTCCAGTGTTGGCCTGGTCGCTTTGA
TTCTTCCTGCACAGCTCAGCCCACCCCATCTGTACTGGAAGGGACCCTGGAGCCCTGGGA
CCACCCCCTTTCAAGTCCCAAACACTGAGTCCTCTGTCATGTGTCCTTGTCCCTAGGCTA
CAATGCTACACTTGGCTCAGGCTGATCACCATGCTAGTCACAGGAGGGAGAGAGAGGCAG
TGTACTTGCCCCCATCTCCTGGAGGGAGGTGCGTTGGAAATTCTAGGCCCTTCAGCAGAG
ACCCATTTGTGCCCAGACCACAATATTTCTTCTTCCTCAGGGACTACATGCCACAGACCT
GTATCCCCAGTGGCAACTCCTGTTAGCTCCCAAACTTAAACAGTGATCTCTTCTAAATAT
ACAAGGCATCTACCCAGCCCCAATAGTGAATAAAAGTCACAAATTTAAGTTCAACACCCT
AAGCAATTTCTCAAAGCCAGTCTACCTGACTAGGTCTATCCACGCACGGTTCCCAACCCC
AAGGGGGAATTAGGGGATTAGATTAAGAGTAGGTAAAGACCCTTTTGTTCTTTCCTGTTG
TAGGGGAGGAAGCTGTTCTCCCCACCCCAGGTGCCTTTGGAGGTTAAGGCTAGGTTAGGC
CCTGCACTCCACCTGGGGACGGCCCCTCATCACCTGAAGTCCTCTGCTGTGTTGACTGGA
CCCTCAGTTCCCACACTGCTAATTACTATGAAAGCTGCTCTGGCCTCGTCTTCTAGCTTG
TGCCTAGTTGAAAGTCTCACCTTTACAGGATCATTTGTAATGCTGGTGTACATGCAGGGA
ACTCACAAGCTGTGTCAGGTATGCAGGACAAGGAATGTCTGCTCTGGGGGGCACAGGCCA
ACTATATGGAGGGAGGGTCCCAAATTAGCAAACCCTTCATTTAAAGCAAACCCTTGTCAA
GCCTACCAGAGCAGCTGATACGTGTGCATAACAGTGAGGCATTGTGTCCTGGATCGTCTG
GGAGCCCAGTGTCAAGTCTGCTGGAATGCAGGAAGTAGAACAGAATCGCCACAGGACTGT
TCTGGGGCCAGCTTCCCTTAACTCTGTAGCCTGGCAGTCTGACCCAAAGTTGCCCTCACC
CAAAGGTTCTGGCTCTTCCCTCCCTCACTTTTACTTTCCCTTCCCCCATAAGTTGGAGGA
TAAAATGGGTATCAATGCTAATATTTCCAGGGAGAACATGAAACCAGAGGTTTCTTTCTT
TCTCTGTAATCTGCTATGAAAAAAAAAAAAAAA
Restriction Sites ECoRI-NOT |
ACCN NM_031899
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_031899.2, NP_114105.1
RefSeq Size 3057 bp
RefSeq ORF 1323 bp
Locus ID 64689
UniProt ID Q9BQQ3
Cytogenetics 3p22.2
Domains GRASP55_65
Summary The Golgi complex plays a key role in the sorting and modification of proteins exported from the endoplasmic reticulum. The protein encoded by this gene is a membrane protein involved in establishing the stacked structure of the Golgi apparatus. It is a caspase-3 substrate, and cleavage of this encoded protein contributes to Golgi fragmentation in apoptosis. This encoded protein can form a complex with the Golgi matrix protein GOLGA2, and this complex binds to the vesicle docking protein p115. Alternative splicing results in multiple transcript variants of this gene. provided by RefSeq, Jul 2013
Transcript Variant: This variant (1) encodes the longest isoform (1).
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"NM_031899" in other vectors (7)
SKU Description Size Price
RC210076 GORASP1 (Myc-DDK-tagged)-Human golgi reassembly stacking protein 1, 65kDa (GORASP1) 10 ug
$457.00
RC210076L1 Lenti ORF clone of Human golgi reassembly stacking protein 1, 65kDa (GORASP1), Myc-DDK-tagged 10 ug
$757.00
RC210076L2 Lenti ORF clone of Human golgi reassembly stacking protein 1, 65kDa (GORASP1), mGFP tagged 10 ug
$757.00
RC210076L3 Lenti ORF clone of Human golgi reassembly stacking protein 1, 65kDa (GORASP1), Myc-DDK-tagged 10 ug
$757.00
RC210076L4 Lenti ORF clone of Human golgi reassembly stacking protein 1, 65kDa (GORASP1), mGFP tagged 10 ug
$757.00
RG210076 GORASP1 (tGFP-tagged) - Human golgi reassembly stacking protein 1, 65kDa (GORASP1) 10 ug
$489.00 $657.00
SC107158 GORASP1 (untagged)-Human golgi reassembly stacking protein 1, 65kDa (GORASP1) 10 ug
$457.00

file-alt Citations

Chinese herbal medicine Fuzheng Kang-Ai decoction sensitized the effect of gefitinib on inhibition of human lung cancer cells through inactivating PI3-K/Akt -mediated suppressing MUC1 expression
Li, Wang, Zheng et al
J Ethnopharmacol (2016) 194, 918-929 DOI: 10.1016/j.jep.2016.10.077
Cited by 27
AI Summary: In this study, OriGene products—specifically the Akt (RC208447), p65 (RC217938), and MUC1 (RC210307) expression plasmids—were used for transient transfection experiments to investigate the roles of these proteins in mediating the effects of Fuzheng Kang-Ai decoction and gefitinib on lung cancer cell growth and signaling pathways.
Showing 1-1 of 1 papers.
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