GRASP65 (GORASP1) (NM_031899) Human Untagged Clone

SKU SC107158

GORASP1 (untagged)-Human golgi reassembly stacking protein 1, 65kDa (GORASP1)

Product Data

• Type: Human Untagged Clone
• Target Symbol: GRASP65
• Synonyms: GOLPH5; GRASP65; P65
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC107158 sequence for NM_031899 edited (data generated by NextGen Sequencing); ATGGGCCTGGGCGTCAGCGCTGAGCAGCCCGCAGGCGGCGCCGAGGGCTTCCACCTCCAC; GGGGTGCAGGAGAACTCCCCAGCCCAGCAGGCGGGCCTGGAGCCCTACTTTGACTTCATC; ATCACCATTGGGCACTCGAGGCTGAACAAGGAGAATGACACCCTGAAGGCACTACTGAAA; GCCAATGTGGAGAAGCCCGTGAAGCTGGAGGTGTTCAATATGAAGACCATGAGGGTGCGC; GAGGTGGAGGTGGTGCCCAGCAACATGTGGGGCGGCCAGGGCCTACTGGGTGCCAGTGTG; CGCTTCTGCAGCTTCCGCAGGGCCAGTGAGCAGGTGTGGCATGTGCTGGATGTGGAACCA; TCTTCACCTGCTGCCCTTGCCGGCCTGCGCCCCTACACAGACTATGTGGTTGGTTCGGAC; CAGATTCTCCAGGAGTCCGAGGACTTCTTTACGCTCATCGAGTCTCATGAGGGGAAGCCC; TTGAAGCTGATGGTGTATAACTCCAAGTCAGACTCCTGCCGGGAGGTGACTGTAACTCCC; AACGCAGCCTGGGGTGGAGAGGGCAGTCTGGGATGTGGCATTGGCTATGGGTATCTACAC; CGGATCCCAACTCAGCCCCCCAGCTACCACAAGAAGCCACCTGGCACCCCACCACCTTCT; GCTCTACCACTTGGTGCCCCACCACCTGATGCTCTACCACCTGGACCCACCCCCGAGGAC; TCTCCTTCCCTGGAGACAGGTTCCAGGCAGAGTGACTACATGGAGGCCCTGCTGCAGGCA; CCTGGCTCCTCCATGGAGGATCCCCTTCCTGGGCCTGGGAGTCCCAGCCACAGTGCTCCA; GACCCTGATGGACTTCCCCATTTCATGGAGACTCCTCTTCAGCCCCCACCTCCAGTGCAG; CGAGTTATGGACCCAGGCTTCCTGGACGTGTCGGGAATTTCTCTCTTGGACAACAGCAAT; GCCAGTGTGTGGCCCAGCCTGCCCTCTTCCACAGAACTGACCACCACAGCTGTCTCAACC; TCAGGGCCAGAGGACATCTGCTCCAGCAGCAGTTCTCATGAGCGGGGTGGTGAGGCTACA; TGGTCTGGGTCAGAGTTTGAGGTCTCCTTCCTGGACAGCCCAGGTGCCCAAGCCCAGGCG; GACCACCTGCCTCAGCTGACTCTTCCTGACAGTCTCACCTCTGCAGCCTCACCAGAAGAT; GGGCTGTCCGCCGAGCTGCTTGAAGCTCAGGCTGAGGAGGAACCAGCAAGCACAGAGGGC; CTAGATACTGGGACGGAGGCTGAGGGGCTGGACAGCCAGGCCCAGATCTCTACCACAGAA; TAA; ; Clone variation with respect to NM_031899.2;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_031899 unedited; CGTTGCGGATTAGTACACTTTCACTATAGGCGGCCGCGAATTCGCAGAGGGCGCAGCGGG; ACTGGACCTGGGTGCCGAGCGGAGCCGCTGCCATGGGCCTGGGCGTCAGCGCTGAGCAGC; CCGCAGGCGGCGCCGAGGGCTTCCACCTCCACGGGGTGCAGGAGAACTCCCCAGCCCAGC; AGGCGGGCCTGGAGCCCTACTTTGACTTCATCATCACCATTGGGCACTCGAGGCTGAACA; AGGAGAATGACACCCTGAAGGCACTACTGAAAGCCAATGTGGAGAAGCCCGTGAAGCTGG; AGGTGTTCAATATGAAGACCATGAGGGTGCGCGAGGTGGAGGTGGTGCCCAGCAACATGT; GGGGCGGCCAGGGCCTACTGGGTGCCAGTGTGCGCTTCTGCAGCTTCCGCAGGGCCAGTG; AGCAGGTGTGGCATGTGCTGGATGTGGAACCATCTTCACCTGCTGCCCTTGCCGGCCTGC; GCCCCTACACAGACTATGTGGTTGGTTCGGACCAGATTCTCCAGGAGTCCGAGGACTTCT; TTACGCTCATCGAGTCTCATGAGGGGAAGCCCTTGAAGCTGATGGTGTATAACTCCAAGT; CAGACTCCTGCCGGGAGGTGACTGTAACTCCCAACGCAGCCTGTGGTGGAGAGGGCAGTC; TGGGATGTGGCATTGGCTATGGGTATCTACACCGGATCCCAACTCAGCCCCCCAGCTACC; ACAAAGAAGCCACCTGGCACCCCACCACCTTCTGCTCTACCACTTGGCTGCCCCACCACC; TGAAGCTTTACCCCCTGGACCCACCCCGGAGGACTTCCCTTTCCTGGAGACAGGTTCCGG; CCGAGTGACTCCTGGAGGCCCTGTTGAGGCACCCGCTCCTCCTGGAGAACCCTTCCTGGC; CTGGGATCCCACC;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_031899 unedited; NAATCTCTGNACCGCAGGCCGCAATCTANGATCGGTTTTTTTTTTTTTTTTTTAAAATAT; TTCAAAGTGTAGTACACATTTATTTTCATTTGTTATTTTCTTTCATAGCAGATTACAGAG; AAAGAAAGAAACCTCTGGTTTCATGTTCTCCCTGGAAATATTAGCATTGATACCCATTTT; ATCCTCCAACTTATGGGGGAAGGGAAAGTAAAAGTGAGGGAGGGAAGAGCCAGAACCTTT; GGGTGAGGGCAACTTTGGGTCAGACTGCCAGGCTACAGAGTTAAGGGAAGCTGGCCCCAG; AACAGTCCTGTGGCGATTCTGTTCTACTTCCTGCATTCCAGCAGACTTGACACTGGGCTC; CCAGACGATCCAGGACACAATGCCTCACTGTTATGCACACGTATCAGCTGCTCTGGTAGG; CTTGACAAGGGTTTGCTTTAAATGAAGGGTTTGCTAATTTGGGACCCTCCCTCCATATAG; TTGGCCTGTGCCCCCCAGAGCAGACATTCCTTGTCCTGCATACCTGACACAGCTTGTGAG; TTCCCTGCATGTACACCAGCATTACAAATGATCCTGTAAAGGTGAGACTTTCAACTAGGC; ACAAGCTAGAAGACGAGGCCAGAGCAGCTTTCATAGTAATTAGCAGTGTGGGAACTGAGG; GTCCAGTCAACACAGCAGAGGACTTCAGGTGATGAGGGGCCGTCCCCAGGTGGAGTGCAG; GCCCTAACCTANCCTTAACCTCCAAAAGCACCTGGGGTGGGAAGAACAGCTTCCTCCCCT; ACACCAGGAAAAACCAAAGGGTCTTTACCTACTTTTAAACTAATCCCCTAATTCCCCTTG; GGGGTGGGGACCCGGCGTGGTTAAACCTAATCCAGTAAACGGCTTTGAAAATTGCTTAAG; GGGGTAACCTAAATTCGGGACCTTCATTCCTTTTGGGGCTGGAAAATA;
• Restriction Sites: ECoRI-NOT
• ACCN: NM_031899
• Insert Size: 2770 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_031899.2, NP_114105.1
• RefSeq Size: 3057 bp
• RefSeq ORF: 1323 bp
• Locus ID: 64689
• UniProt ID: Q9BQQ3
• Cytogenetics: 3p22.2
• Domains: GRASP55_65
• Summary: The Golgi complex plays a key role in the sorting and modification of proteins exported from the endoplasmic reticulum. The protein encoded by this gene is a membrane protein involved in establishing the stacked structure of the Golgi apparatus. It is a caspase-3 substrate, and cleavage of this encoded protein contributes to Golgi fragmentation in apoptosis. This encoded protein can form a complex with the Golgi matrix protein GOLGA2, and this complex binds to the vesicle docking protein p115. Alternative splicing results in multiple transcript variants of this gene. [provided by RefSeq, Jul 2013]; Transcript Variant: This variant (1) encodes the longest isoform (1).