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ULK3 (NM_001099436) Human Untagged Clone

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SKU
SC323667
ULK3 (untagged)-Kinase deficient mutant (K87M) of Human unc-51-like kinase 3 (C. elegans) (ULK3)
  $457.00
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol ULK3
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene ORF within SC323667 sequence for NM_001099436 edited (data generated by NextGen Sequencing)
ATGGCGGGGCCCGGCTGGGGTCCCCCGCGCCTGGACGGCTTCATCCTCACCGAGCGCCTG
GGCAGCGGCACGTACGCCACGGTGTACAAGGCCTACGCCAAGAAGGACACTCGTGAAGTG
GTAGCCATAATGTGTGTAGCCAAGAAAAGTCTGAACAAGGCATCGGTGGAGAACCTCCTC
ACGGAGATTGAGATCCTCAAGGGCATTCGACATCCCCACATTGTGCAGCTGAAAGACTTT
CAGTGGGACAGTGACAATATCTACCTCATCATGGAGTTTTGCGCAGGGGGCGACCTGTCT
CGCTTCATCCATACCCGCAGGATTCTGCCTGAGAAGGTGGCGCGTGTCTTCATGCAGCAA
TTAGCTAGCGCCCTGCAATTCCTGCATGAACGGAATATCTCTCACCTGGATCTGAAGCCA
CAGAACATTCTACTGAGCTCCTTGGAGAAGCCCCACCTAAAACTGGCAGACTTTGGTTTC
GCACAACACATGTCCCCGTGGGATGAGAAGCACGTGCTCCGTGGCTCCCCCCTCTACATG
GCCCCCGAGATGGTGTGCCAGCGGCAGTATGACGCCCGCGTGGACCTCTGGTCCATGGGG
GTCATCCTGTATGAAGCCCTCTTCGGGCAGCCCCCCTTTGCCTCCAGGTCGTTCTCGGAG
CTGGAAGAGAAGATCCGTAGCAACCGGGTCATCGAGCTCCCCTTGCGGCCCCTGCTCTCC
CGAGACTGCCGGGACCTACTGCAGCGGCTCCTGGAGCGGGACCCCAGCCGTCGCATCTCC
TTCCAGGACTTTTTTGCGCACCCCTGGGTGGACCTGGAGCACATGCCCAGTGGGGAGAGT
CTGGGGCGAGCAACCGCCCTGGTGGTGCAGGCTGTGAAGAAAGACCAGGAGGGGGATTCA
GCAGCTGCCTTATCACTCTACTGCAAGGCTCTGGACTTCTTTGTACCTGCCCTGCACTAT
GAAGTGGATGCCCAGCGGAAGGAGGCAATTAAGGCAAAGGTGGGGCAGTACGTGTCCCGG
GCTGAGGAGCTCAAGGCCATCGTCTCCTCTTCCAATCAGGCCCTGCTGAGGCAGGGGACC
TCTGCCCGAGACCTGCTCAGAGAGATGGCCCGGGACAAGCCACGCCTCCTAGCTGCCCTG
GAAGTGGCTTCAGCTGCCATGGCCAAGGAGGAGGCCGCCGGCGGGGAGCAGGATGCCCTG
GACCTGTACCAGCACAGCCTGGGGGAGCTACTGCTGTTGCTGGCAGCGGAGCCCCCGGGC
CGGAGGCGGGAGCTGCTTCACACTGAGGTTCAGAACCTCATGGCCCGAGCTGAATACTTG
AAGGAGCAGGTCAAGATGAGGGAATCTCGCTGGGAAGCTGACACCCTGGACAAAGAGGGA
CTGTCGGAATCTGTTCGTAGCTCTTGCACCCTTCAGTGA

Clone variation with respect to NM_001099436.1
131 a=>t
5' Read Nucleotide Sequence
>OriGene 5' read for mutant NM_001099436 unedited
CCCGCCCGTCCCGCAACGGGCGGTAGGCGCTGTACGGTTGGGAGGTTCATATAAGCAGAGCTCGTTTAGT
GAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGCCGCGCCTGGAC
GGCTTCATCCTCACCGAGCGCCTGGGCAGCGGCACGTACGCCACGGTGTACAAGGCCTACGCCAAGAAGG
ACACTCGTGAAGTGGTAGCCATAATGTGTGTAGCCAAGAAAAGTCTGAACAAGGCATCGGTGGAGAACCT
CCTCACGGAGATTGAGATCCTCAAGGGCATTCGACATCCCCACATTGTGCAGCTGAAAGACTTTCAGTGG
GACAGTGACAATATCTACCTCATCATGGGAGTTTTGCGCAGGGGGCGACCCTGTCTCGCTTCATCCCATA
CCCCGCAGGATTTCTGCCTGAAAAGGTGCCGCGGTGTCTTCATGCAGCATTTAGCTAGCCGCCCCTGCAT
TCCTGCATGAAACGGAATTATCCTCTCACCTGATCCGGAGCCCCAGAACATCTACTGACCTCCTGGAAAA
GCCCACCTAAAACTGGCAAACTTTGGTTTGCAACACACAGGTCCCTTGGTAGAAGCCTTCCCCGGGTCTC
CCCTCTAATGCCCCAATTGGTGCCACGCGATTAGACCCCTTGACTTTGTCCAGGGGGCTCTCGTGTAAGC
CTTTGGCAGCCCTTGCCCAGTGTCTCTGACTGAAGAATCTGAAACGGTATAGTCCTTGGCCTGTTCGAAT
CGCACTCGACGCCTGCGGACAGCGTATATTGATTGACTGACGATCCTTGGACTGCAACCGTTGACGA
Kinase Domain Sequence
>SC323667 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
CTCTGMGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAACCGTC
AGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGCCGCGCCTGGACGGCTTCAT
CCTCACCGAGCGCCTGGGCAGCGGCACGTACGCCACGGTGTACAAGGCCTACGCCAAGAAGGACACTCGT
GAAGTGGTAGCCATAATGTGTGTAGCCAAGAAAAGTCTGAACAAG
Restriction Sites Please inquire |
ACCN NM_001099436
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001099436.1, NP_001092906.1
RefSeq Size 2635 bp
RefSeq ORF 1419 bp
Locus ID 25989
UniProt ID Q6PHR2
Cytogenetics 15q24.1
Protein Families Druggable Genome, Protein Kinase
Protein Pathways mTOR signaling pathway, Regulation of autophagy
Summary Serine/threonine protein kinase that acts as a regulator of Sonic hedgehog (SHH) signaling and autophagy. Acts as a negative regulator of SHH signaling in the absence of SHH ligand: interacts with SUFU, thereby inactivating the protein kinase activity and preventing phosphorylation of GLI proteins (GLI1, GLI2 and/or GLI3). Positively regulates SHH signaling in the presence of SHH: dissociates from SUFU, autophosphorylates and mediates phosphorylation of GLI2, activating it and promoting its nuclear translocation. Phosphorylates in vitro GLI2, as well as GLI1 and GLI3, although less efficiently. Also acts as a regulator of autophagy: following cellular senescence, able to induce autophagy.UniProtKB/Swiss-Prot Function
Transcript Variant: This variant (1) encodes the longest isoform (a).
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"NM_001099436" in other vectors (7)
SKU Description Size Price
RC220209 ULK3 (Myc-DDK-tagged)-Human unc-51-like kinase 3 (C. elegans) (ULK3) 10 ug
$457.00
RC220209L1 Lenti ORF clone of Human unc-51-like kinase 3 (C. elegans) (ULK3), Myc-DDK-tagged 10 ug
$757.00
RC220209L2 Lenti ORF clone of Human unc-51-like kinase 3 (C. elegans) (ULK3), mGFP tagged 10 ug
$757.00
RC220209L3 Lenti ORF clone of Human unc-51-like kinase 3 (C. elegans) (ULK3), Myc-DDK-tagged 10 ug
$757.00
RC220209L4 Lenti ORF clone of Human unc-51-like kinase 3 (C. elegans) (ULK3), mGFP tagged 10 ug
$757.00
RG220209 ULK3 (tGFP-tagged) - Human unc-51-like kinase 3 (C. elegans) (ULK3) 10 ug
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SC316533 ULK3 (untagged)-Human unc-51-like kinase 3 (C. elegans) (ULK3) 10 ug
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