DNAJB4 (NM_007034) Human Untagged Clone

SKU SC322522

DNAJB4 (untagged)-Human DnaJ (Hsp40) homolog, subfamily B, member 4 (DNAJB4)

Product Data

• Type: Human Untagged Clone
• Target Symbol: DNAJB4
• Synonyms: DjB4; DNAJW; HLJ1
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for SC322522 GAGACGCTGTCTGCTTGCTGCCTTAAGACAGCTAGCTGAATTGCTGATTAACTTTTAAAA; TACCCAGCTTGGTTTATTTTTCTTAGAATCTGTTGCTAAGACTGGGGACGCTGTTTTCTT; TTACAAAGGGAAATCTAAGTTAATTTCAAGGCATTCGAAATGGGGAAAGACTATTATTGC; ATTTTGGGAATTGAGAAAGGAGCTTCAGATGAAGATATTAAAAAGGCTTACCGAAAACAA; GCCCTCAAATTTCATCCGGACAAGAACAAATCTCCTCAGGCAGAGGAAAAATTTAAAGAG; GTCGCAGAAGCTTATGAAGTATTGAGTGATCCTAAAAAGAGAGAAATATATGATCAGTTT; GGGGAGGAAGGGTTGAAAGGAGGAGCAGGAGGTACTGATGGACAAGGAGGTACCTTCCGG; TACACCTTTCATGGCGATCCTCATGCTACATTTGCTGCATTTTTCGGAGGGTCCAACCCC; TTTGAAATTTTCTTTGGAAGACGAATGGGTGGTGGTAGAGATTCTGAAGAAATGGAAATA; GATGGTGATCCTTTTAGTGCCTTTGGTTTCAGCATGAATGGATATCCAAGAGACAGGAAT; TCTGTGGGGCCATCCCGCCTCAAACAAGATCCTCCAGTTATTCATGAACTTAGAGTATCA; CTTGAAGAGATATATAGTGGTTGTACCAAACGGATGAAGATTTCTCGAAAAAGGCTAAAC; GCTGATGGAAGGAGTTACAGATCTGAGGACAAAATTCTTACCATTGAGATTAAAAAAGGG; TGGAAAGAAGGCACCAAAATTACTTTTCCAAGAGAAGGAGATGAAACACCAAATAGTATT; CCAGCAGACATTGTTTTTATCATTAAAGACAAAGATCATCCAAAATTTAAAAGGGATGGA; TCAAATATAATTTATACTGCTAAAATTAGTTTACGAGAGGCATTGTGTGGCTGCTCAATT; AATGTACCAACACTGGATGGAAGAAACATACCTATGTCAGTAAATGATATTGTGAAACCC; GGAATGAGGAGAAGAATTATTGGATATGGGCTGCCATTTCCAAAAAATCCTGACCAACGT; GGTGACCTTCTAATAGAATTTGAGGTGTCCTTCCCAGATACTATATCTTCTTCATCCAAA; GAAGTACTTAGGAAACATCTTCCTGCCTCATAGAATGAAGAACTTTGTTACACATATTTT; GATAAGGCACTGAAAATATAAAAGGACTGGTAGTTTACTGATGTAGATGTGAATTCTGTA; TAAAGATGTGTAAATTCTTTTGAGGGTTCATTAAATTGCATGAATAGAGACGGGTCAAAT; AAATAGGCAAAAGGGATTTTTACAGTTAGAGATAAAAGAGAAAACCATTCACTGTATTTT; ATTTCATTTCTCCTGATTCAGATATTTTTAGTAATTTGCTTATATGTAAAAGTTGTTTTT; GTGGAGTCAGTGGATATATTTCTAATGAAGTGCTAGACTATCCAATTACTTAATTTCTTA; TACCTTTAGATAATCAGTATGAAAAGTTCCCATTTATAATGGAAATGAAAATTCTTAACT; AAACTATACATGTAATATGTATTTCTAGAAGAGAATAAAAACCCAAGTCAGTTATTAGAT; TTAAATCACCTTCTGAAATGCTGCTATAGGGCTGGTATCTGTAAAAGAATATCCTGATGC; ATCTGTTTCACCATTTTGATTTTTAAAGTATGCTGTAGCATTTCTTAATAACATCGTTGT; GATGTTCTTAAGGCAGATCTTTCTTCATAAAAAGGAAAGTAATGGCAATTTCTCTCCTGT; GGAAATCCCAATTGCTTGAATTACTGATATTTTAGAATAGACTTTTTAAAATGCCATATG; TAATTTTATGCAAGTTGACTATATATCTTGTACTTAATAAATTATAGGCTCATTTTGTTC; TCTGCTAGTTTAAAGTAATTCGTTTAATAATAGATGTGTTTTTAGAGGAAATGCTGTTAC; TTGGAATTAATTTTCCAGTTATACAGTCTTCTATAACTTACTAATAATATTCTATATGTA; CTTTATGTAATTTCCCTAAAAAGAATGAACTACCACTACACTATGGTGTTAAACCAAAAT; ATAGGGAAAATAAACACTAACTGCTGCTTATGGATAATGTTGCAACTACTTGTTATGCAT; ATAAATATTTTACTTTTTCACATGTATAGATTGCATTTCTTAGGTGTTTTAATTTTTTAA; ATATATTTATGTTTTAAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_007034
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_007034.3, NP_008965.2
• RefSeq Size: 2250 bp
• RefSeq ORF: 1014 bp
• Locus ID: 11080
• UniProt ID: Q9UDY4
• Cytogenetics: 1p31.1
• Domains: DnaJ, DnaJ_C
• Summary: The protein encoded by this gene is a molecular chaperone, tumor suppressor, and member of the heat shock protein-40 family. The encoded protein binds the cell adhesion protein E-cadherin and targets it to the plasma membrane. This protein also binds incorrectly folded E-cadherin and targets it for endoplasmic reticulum-associated degradation. This gene is a strong tumor suppressor for colorectal carcinoma, and downregulation of it may serve as a good biomarker for predicting patient outcomes. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2015]; Transcript Variant: This variant (1) encodes the longest isoform (a). Variants 1 and 2 both encode the same isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.