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PPM1F (NM_014634) Human Untagged Clone

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SKU
SC320526
PPM1F (untagged)-Human protein phosphatase, Mg2+/Mn2+ dependent, 1F (PPM1F)
  $457.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol PPM1F
Synonyms CAMKP; CaMKPase; FEM-2; hFEM-2; POPX2
Vector pCMV6-AC
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>OriGene sequence for NM_014634.2 GAGGAGACAGCTGAGGCCCGCGGAGACCAGGGGGTGAAGCCTGGAGACCCTCTTGCCCTG
GCCTAGCTGCAGGCCCCCGGGATGCTTTGGGCATGTCCTCTGGAGCCCCACAGAAGAGCA
GCCCAATGGCCAGTGGAGCTGAGGAGACCCCAGGCTTCCTGGACACGCTCCTGCAAGACT
TCCCAGCCCTGCTGAACCCAGAGGACCCTCTGCCATGGAAGGCCCCAGGGACGGTGCTCA
GCCAGGAGGAGGTGGAGGGCGAGCTGGCTGAGCTGGCCATGGGCTTTCTGGGCAGCAGGA
AGGCCCCGCCACCACTTGCTGCTGCTCTGGCCCACGAAGCAGTTTCACAGCTGCTACAGA
CAGACCTTTCCGAATTCAGGAAGTTGCCCAGGGAGGAAGAAGAAGAGGAGGAGGACGATG
ACGAGGAAAAGGCCCCTGTGACCTTGCTGGATGCCCAAAGCCTGGCACAGAGTTTCTTTA
ACCGCCTTTGGGAAGTCGCCGGCCAGTGGCAGAAGCAGGTGCCATTGGCTGCCCGGGCCT
CACAGCGGCAGTGGCTGGTCTCCATCCACGCCATCCGGAACACTCGCCGCAAGATGGAGG
ACCGGCACGTGTCCCTCCCTTCCTTCAACCAGCTCTTCGGCTTGTCTGACCCTGTGAACC
GCGCCTACTTTGCTGTGTTTGATGGTCACGGAGGCGTGGATGCTGCGAGGTACGCCGCTG
TCCACGTGCACACCAACGCTGCCCGCCAGCCAGAGCTGCCCACAGACCCTGAGGGAGCCC
TCAGAGAAGCCTTCCGGCGCACCGACCAGATGTTTCTCAGGAAAGCCAAGCGAGAGCGGC
TGCAGAGCGGCACCACAGGTGTGTGTGCGCTCATTGCAGGAGCGACCCTGCACGTCGCCT
GGCTCGGGGATTCCCAGGTCATTTTGGTACAGCAGGGACAGGTGGTGAAGCTGATGGAGC
CACACAGACCAGAACGGCAGGATGAGAAGGCGCGCATTGAAGCATTGGGTGGCTTTGTGT
CTCACATGGACTGCTGGAGAGTCAACGGGACCCTGGCCGTCTCCAGAGCCATCGGGGATG
TCTTCCAGAAGCCCTACGTGTCTGGGGAGGCCGATGCAGCTTCCCGGGCGCTGACGGGCT
CCGAGGACTACCTGCTGCTTGCCTGTGATGGCTTCTTTGACGTCGTACCCCACCAGGAAG
TTGTTGGCCTGGTCCAGAGCCACCTGACCAGGCAGCAGGGCAGCGGGCTCCGTGTCGCCG
AGGAGCTGGTGGCTGCGGCCCGGGAGCGGGGCTCCCACGACAACATCACGGTCATGGTGG
TCTTCCTCAGGGACCCCCAAGAGCTGCTGGAGGGCGGGAACCAGGGAGAAGGGGACCCCC
AGGCAGAAGGGAGGAGGCAGGACTTGCCCTCCAGCCTTCCAGAACCTGAGACCCAGGCTC
CACCAAGAAGCTAGGTGGTTTCCAGGCCCCTGCCCTCCCCTTCCTCCCATCCTTGTCCTT
CTCTCCCTCAGAAGCCTCAGGACCCAACAGGTGGCAGGCAGTGGACAGGGTGCCCGCCCC
ACAGTGCTTTCCCCAGCACCCCAGAGCCAGTCGGGACACCCCCCGCAGCCCGTCCTGGTG
GCTGTGGAACTGCACTGGGTGGCGGGCAGATGGTGGAAGGCAGCTTAGGAGACCTCACCA
AAGAGAAGATGGACCGGCTCTTGCTCCCAGCTCCTATTAGGCCCGGGGTGGGACCAGAGG
TCATAGGTGCCCAACGGCAGCCAAACCAAAGACACTGGTGTGCATGGGGCAGCATGGTTG
TGCACGTGGGACCCTGGGGCGGACCCAGGAGCCAAACTCTTGAAGCACCCCCTGGGTCAG
GCCCAGCAGCGGAGTGGCCAGCCCCAGTTTCCCATTGCTCCTCTCTGCGGCCAGGGCCAG
GTGGGTTCATATTTACAGATATGCCCAGCCAGTCCTGGTCGGCCACACCAGTGTCCCAAA
GAGGAGAGCGCAGCAGAGCCAGGGGTCTGTTCTGTAGCAGCCACCCCCCTGCCCCCACTC
CAGGGCAGCCATGATGTGCTTGGGCCCACCAGGGCCTTCCGGGCTGCTCTCTTCCCTGAG
CCCGGAACCGGCGACGCACATGTGTCTTTTGTTGGTGTGTTTGTTTTTTTCCAGGGAGGT
CTAATTCCGAAGCAGTATTCCAGGTTTTCTCTTTGTTTTATCAGTGCCAAGATGACCTGT
TGTGTCATATAATTTAAGCAGAGCTTAGCATTTATTTTATTCTTTAGAAAACTTAAGTAT
TTACTTTTTTAAAGCTATTTTTCAAGGAAAAAAAAAAAAAAAAAAAAA
Restriction Sites Please inquire |
ACCN NM_014634
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_014634.2, NP_055449.1
RefSeq Size 5169 bp
RefSeq ORF 1365 bp
Locus ID 9647
UniProt ID P49593
Cytogenetics 22q11.22
Domains PP2C
Protein Families Druggable Genome, Phosphatase
Summary The protein encoded by this gene is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase can interact with Rho guanine nucleotide exchange factors (PIX), and thus block the effects of p21-activated kinase 1 (PAK), a protein kinase mediating biological effects downstream of Rho GTPases. Calcium/calmodulin-dependent protein kinase II gamma (CAMK2G/CAMK-II) is found to be one of the substrates of this phosphatase. The overexpression of this phosphatase or CAMK2G has been shown to mediate caspase-dependent apoptosis. An alternatively spliced transcript variant has been identified, but its full-length nature has not been determined. provided by RefSeq, Jul 2008
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Documents
Resources
"NM_014634" in other vectors (7)
SKU Description Size Price
RC210043 PPM1F (Myc-DDK-tagged)-Human protein phosphatase, Mg2+/Mn2+ dependent, 1F (PPM1F) 10 ug
$1,025.00
RC210043L1 Lenti ORF clone of Human protein phosphatase, Mg2+/Mn2+ dependent, 1F (PPM1F), Myc-DDK-tagged 10 ug
$1,325.00
RC210043L2 Lenti ORF clone of Human protein phosphatase, Mg2+/Mn2+ dependent, 1F (PPM1F), mGFP tagged 10 ug
$1,325.00
RC210043L3 Lenti ORF clone of Human protein phosphatase, Mg2+/Mn2+ dependent, 1F (PPM1F), Myc-DDK-tagged 10 ug
$1,325.00
RC210043L4 Lenti ORF clone of Human protein phosphatase, Mg2+/Mn2+ dependent, 1F (PPM1F), mGFP tagged 10 ug
$1,325.00
RG210043 PPM1F (tGFP-tagged) - Human protein phosphatase, Mg2+/Mn2+ dependent, 1F (PPM1F) 10 ug
$489.00 $1,225.00
SC114924 PPM1F (untagged)-Human protein phosphatase, Mg2+/Mn2+ dependent, 1F (PPM1F) 10 ug
$457.00

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